中国药理学通报2017，Vol.33Issue(8)：1109-1113,5.DOI:10.3969/j.issn.1001-1978.2017.08.014
HMGB1和TNFAIP3在狼疮性肾炎系膜细胞增殖中的作用
Role of HMGB1 and TNFAIP3 involvedin mesangial cell proliferation of lupus nephritis
摘要
Abstract
Aim To investigate the mechanism of high mobility group protein B1(HMGB1)and tumor necrosis factor α-induced protein-3(TNFAIP3)involved in cell proliferation in lupus nephritis(LN)patients and human mesangial cells(HMC).Methods Immunofluorescence and immunohistochemistry technique were employed to detect HMGB1,TNFAIP3 and IκBα expression levels in glomerular cells of type Ⅳ LN patients.BrdU incorporation technology was used to detect cell proliferation level in HMC after stimulated by recombinant HMGB1.TNFAIP3 and IκBα expression levels in HMC were detected after HMGB1 stimulation by Western blot.Results The expression levels of HMGB1 and TNFAIP3 were increased in LN patients,while IκBα was decreased.HMC proliferation levels increased significantly after HMGB1 stimulation.At the same time,30 minutes after HMGB1 stimulation,the expression level of TNFAIP3 was significantly increased(P<0.05),while IκBα decreased(P<0.05)and then p65 increased significantly(P<0.05),compared with control group.Conclusion HMGB1 and TNFAIP3 are probably involved in mesangial cell proliferation by activating of NF-κB signaling pathways in LN pathogenesis.关键词
狼疮性肾炎/HMGB1/TNFAIP3/细胞增殖/人系膜细胞/蛋白表达Key words
lupus nephritis/HMGB1/TNFAIP3/cell proliferation/human mesangial cells/protein expression分类
医药卫生引用本文复制引用
张玮,杨冉,郭惠芳,封晓娟,魏群,张玉,王奕卓,李金泽,刘淑霞..HMGB1和TNFAIP3在狼疮性肾炎系膜细胞增殖中的作用[J].中国药理学通报,2017,33(8):1109-1113,5.基金项目
河北省自然科学基金青年基金资助项目(No H2015206449) （No H2015206449）
河北省卫生厅重点科技研究计划(No ZD20140034) （No ZD20140034）
河北省大学生创新创业训练计划(No 201510089038) （No 201510089038）
