中国药理学通报2017，Vol.33Issue(8)：1126-1131,6.DOI:10.3969/j.issn.1001-1978.2017.08.017
FABP4在妊娠期高血压疾病胎盘滋养细胞免疫炎症反应中的作用研究
Effect of FABP4 on placental trophoblastic immune inflammatory response in patients of hypertensive disorder complicating pregnancy
摘要
Abstract
Aim To investigate the role of fatty acid binding protein 4 (FABP4) in placental trophoblastic immune response in patients with hypertensive disorder complicating pregnancy. Methods The human placental trophoblast cell line (HTR-8) was cultured in vitro and incubated with L-NAME for 0, 10, 100, 500 and 1 000 μmol·L -1 for 48 h. The levels of IL-6 and TNF-α were analysed by ELISA. The Mrna and protein expression levels of FABP4 were detected by Western blot and Qrt-PCR. The cells transfected FABP4 adenovirus expression vector were intervented with 100 μmol·L -1 L-NAME. Then, the levels of IL-6 and TNF-α were determined by ELISA. Results Com- pared with the control group, the expression levels of IL-6, TNF-α and FABP4 were significantly increased in the intervention group(P<0.01). Meanwhile, the expression levels of IL-6 and TNF-α were significantly increased in the overexpressed FABP4 group, compared with the control group(P<0.01). Conclusion FABP4 is involved in regulating the trophoblastic immune-inflammatory response in patients of hypertensive disorder complicating pregnancy.关键词
脂肪酸结合蛋白4/妊娠期高血压疾病/滋养细胞/IL-6/TNF-α/N-硝基-L-精氨酸甲酯Key words
fatty acid binding protein 4/hypertensive disorder of pregnancy/trophoblast/IL-6/TNF-α/N-nitro-L-arginine methylester分类
医药卫生引用本文复制引用
毛彩艳,姜怡邓,张辉,李旭生,杨松昊,邓梅,丁宁,吴凯,杨晓玲,张慧萍..FABP4在妊娠期高血压疾病胎盘滋养细胞免疫炎症反应中的作用研究[J].中国药理学通报,2017,33(8):1126-1131,6.基金项目
国家自然科学基金资助项目(No 81570452,81660258) （No 81570452,81660258）
宁夏高等学校科学研究项目(No NGY2016129,NGY2016086) （No NGY2016129,NGY2016086）
