中国药理学通报2017，Vol.33Issue(8)：1131-1135,5.DOI:10.3969/j.issn.1001-1978.2017.08.018
咪达唑仑对离体猪冠脉环舒张功能的影响及其机制研究
Vasodilatory effect of midazolam on pre-contractions of in-vitro porcine coronary artery and its mechanisms
摘要
Abstract
Aim To investigate the effects of midazolam on porcine isolated coronary artery rings pre-contracted by potassium chloride(KCl)and the possible mechanism.Methods The vessel tension recorder system was used.Isotonic tension of porcine isolated coronary artery rings precontracted by KCl(30 mmol·L-1)was recorded.The vasorelaxing action of midazolam and effects of various drugs were observed in the rings.Results Midazolam(3×10-6～1×10-4 mol·L-1)respectively concentration-dependently reduced the contraction induced by KCl,and there was significant difference between the rings with intact and denude endothelium(P<0.05).On KCl-induced precontraction,midazolam′s relaxation was depressed by L-NAME and the blend of L-NAME and L-Arg(P<0.05),but was not affected by Indo,L-Arg and 1400W.The contraction was not prevented by pretreatment with the inhibitor of Na+/Ca2+ exchanger(KB-R7943).The inhibitor of KATP(Gli)restrained the diastolic function of midazolam(P<0.05),while the inhibitor of BKCa(TEA),Kir(BaCl2),KV(4-AP)had no obvious effect.Conclusions Midazolam produces remarkable vasodilatation on KCl pre-contracted porcine isolated coronary artery rings.Its relaxtion effect is via concentration-dependent and endothelium-dependent mechanisms and relevant to the production of NO.Na+/Ca2+ exchanger is not involved midazolam′s vasodilatation on KCl pre-contracted porcine coronary artery rings.The relaxant mechanism of midazolam may be concerned with KATP.The Kir,BKCa and KV may be not involved.关键词
咪达唑仑/猪冠状动脉环/舒张作用/NO/内皮/钾通道Key words
midazolam/porcine coronary artery rings/vasodilator effect/NO/endothelium/potassium channel分类
医药卫生引用本文复制引用
任俊杰,王科科,许慧玉,黄婷娟,吕怡,武冬梅,张轩萍,郑晓俊..咪达唑仑对离体猪冠脉环舒张功能的影响及其机制研究[J].中国药理学通报,2017,33(8):1131-1135,5.基金项目
山西省研究生教育创新项目(No 2016BY077) （No 2016BY077）
山西医科大学青年基金(No 02201514) （No 02201514）
