中国药理学通报2017，Vol.33Issue(8)：1147-1152,6.DOI:10.3969/j.issn.1001-1978.2017.08.021
马鞭草总黄酮靶向拓扑异构酶Ⅱ诱导肝癌HepG-2细胞凋亡
Apoptosis of human hepatocellular carcinomaHepG-2 cells induced by total flavonoids of Verbena officinalis L through targeting topoisomerase Ⅱ
摘要
Abstract
Aim To explore whether total flavonoids of Verbena officinalis L(TFV)can induce apoptosis of HepG-2 cells through targeting topoisomerase Ⅱ.Methods HepG-2 cells were cultured with TFV at 200,100,50 mg·L-1.Cell apoptosis was evaluated by TUNEL-DAPI double staining.TopⅡ activity was detected by supercoiled pBR322 DNA relaxation assay.The levels of the mRNA of TOP Ⅱα,TOP Ⅱβ were analyzed by real time PCR.Expression of Bcl-2,Bax,TOP Ⅱα,TOP Ⅱβ and caspase-3 was analyzed by Western blot.关键词
马鞭草总黄酮/HepG-2/细胞凋亡/TopⅡ/Bcl-2/Bax/caspase-3信号通路Key words
total flavonoids of Verbena officinalis L/HepG-2/cell apoptosis/TopⅡ/Bcl-2/Bax/caspase-3 signaling pathway分类
医药卫生引用本文复制引用
李先佳,任丽平,金少举..马鞭草总黄酮靶向拓扑异构酶Ⅱ诱导肝癌HepG-2细胞凋亡[J].中国药理学通报,2017,33(8):1147-1152,6.基金项目
河南省科技攻关计划(社会发展领域)(No 162102310596) （社会发展领域）
河南省高等学校青年骨干教师资助计划项目(No 2015GGJS-288) （No 2015GGJS-288）
漯河医学高等专科学校基础科学研究项目(No 2015-S-LMC03) （No 2015-S-LMC03）
