中国药理学通报2017，Vol.33Issue(8)：1170-1175,6.DOI:10.3969/j.issn.1001-1978.2017.08.025
RCP与G蛋白偶联在静压力促血管平滑肌细胞增殖中的作用
Interacting of receptor component protein and G protein in static pressure-induced proliferation of VSMC
摘要
Abstract
Aim To explore the effect of receptor component protein(RCP)in the signal transduction of vascular smooth muscle cell(VSMC)proliferation induced by static pressure.Methods The mouse-derived vascular smooth muscle cell line(A10VSMC)was employed in the experiment.Cells were exposed to static pressure,and MTT assay was used to detect the cell viability.Western blot was used to determine the expressions of PCNA,RCP and p-Akt,RCP mRNA was tested by RT-PCR,and co-immunoprecipitation was used to test the interaction between RCP and G proteins.Results The cell viability,expressions of PCNA and RCP increased with the elevation of static pressure and reached their peaks at 120 mmHg,and after 6 hours they got a plateau.The static pressure significantly increased the level of p-Akt,meanwhile,the binding of RCP and Gαs significantly decreased.However,the binding of RCP and Gβ increased in response to static pressure after stimuli of static pressure,but Gγ was obscure.Conclusion Static pressure can induce VSMC proliferation and expression of RCP,which may involve G protein signal transduction model.关键词
受体组分蛋白/静压力/血管平滑肌细胞/增殖/G蛋白/信号转导Key words
RCP/static pressure/vascular smooth muscle cells/proliferation/G protein/signal transduction分类
医药卫生引用本文复制引用
张晓一,王珍,郭峰,刘玉环,杨丽,阳芳,秦旭平..RCP与G蛋白偶联在静压力促血管平滑肌细胞增殖中的作用[J].中国药理学通报,2017,33(8):1170-1175,6.基金项目
国家自然科学基金资助项目(No 30572192,81173060) （No 30572192,81173060）
南华大学研究生科研创新项目(No 2011XCX13,0223-0002-00031) （No 2011XCX13,0223-0002-00031）
