中国药理学通报2017，Vol.33Issue(8)：1176-1181,6.DOI:10.3969/j.issn.1001-1978.2017.08.026
补阳还五汤精简方对大脑中动脉阻塞模型大鼠海马组织Cdk5的调控
Regulation of thin recipe of Buyang Huanwudecoction on Cdk5 expression of rats after cerebral ischemia
摘要
Abstract
Aim To evaluate the regulation of thin recipe of Buyang Huanwu decoction on cyclin-dependent kinase 5(Cdk5)expressions in hippocampus tissue of rats after cerebral ischemia.Methods Male SD rats were divided into sham-operation group,MCAO group,Buyang Huanwu decoction group(ig.3.15 g·kg-1)and its thin recipe composition group(ig.2.41 g·kg-1).Each group was then divided into five subgroups based on the time after administration for 1,3,7,14,28 d respectively.Cdk5 protein and mRNA levels in each group were examined by using immunohistochemistry,Western blot and real-time PCR respectively.Results The up-regulation of Cdk5 was observed in model rat hippocampus after cerebral ischemia 1 day,and kept increasing with the aggravation of ischemia injury,the peaked expression was observed after 7～14 d,while the downtrend was observed after 28 days compared with the corresponding sham-operation groups(P<0.01),suggesting that the Cdk5 signal pathway would be activated by cerebral ischemic injury.The expression of Cdk5 in thin recipe of Buyang Huanwu decoction group was significantly lower than that in model group at each time point(P<0.05),and there was also more obvious down-regulation with the extend of intervene time.The regulation effects of thin recipe of Buyang Huanwu decoction was up to the best after 28 days of administration,which indicated the thin recipe was positive to the abnormal expression of Cdk5,and there was no difference between Buyang Huanwu decoction and its thin recipe treated groups(P>0.05).Conclusion The thin recipe of Buyang Huanwu decoction could exert the protective effect by regulating Cdk5 after cerebral ischemia.关键词
补阳还五汤/补阳还五汤精简方/大脑中动脉阻塞模型/细胞周期素依赖性蛋白激酶5/脑缺血/海马组织Key words
Buyang Huanwu decoction/thin recipe of Buyang Huanwu decoction/middle cerebral artery occlusion(MCAO)/cyclin-dependent kinase 5(Cdk5)/cerebral ischemia/hippocampus分类
医药卫生引用本文复制引用
刘芳,王宇红,邵乐,夏相宜,佘颜,蔡光先..补阳还五汤精简方对大脑中动脉阻塞模型大鼠海马组织Cdk5的调控[J].中国药理学通报,2017,33(8):1176-1181,6.基金项目
国家重点基础研究发展计划资助项目(No 2012CB723503) （No 2012CB723503）
湖南省中医药科研计划项目(No 2014135) （No 2014135）
湖南省十二五中药学重点学科资助 （）
