中国药业2017，Vol.26Issue(13)：24-27,4.DOI:10.3969/j.issn.1006-4931.2017.13.007
一测多评法测定沙棘鲜果中槲皮素、山柰素和异鼠李素含量
Content Determination of Quercetin, Kaempferide and Isohamnetin in Hippophae Rhamnoides L. by Quantitative Analysis of Multi-Components by Single Marker
摘要
Abstract
Objective To establish a quantitative analysis of multi-components by single marker ( QAMS ) method for determing the con-tent of quercetin, kaempferide, isohamnetin in Hippophae Rhamnoides L., and test the application value of QAMS in the quality control of Hippophae Rhamnoides L. . Methods The Agilent C18 column ( 250 mm × 4. 6 mm, 5 μm ) was adopted, the mobile phase was methol-0. 4% phosphoric acid solution ( 56 :44 ) , the flow rate was 1. 0 mL/min, the column temperature was 30 ℃, the injection volume was 10 μL, and the detection wavelength was set at 370 nm. By using quercetin as reference, the relative correction factors ( RCF ) of kaempferol and isorhmnetin were calculated. The method was evaluated by comparison of the quantitative results through external stan-dard method and QAMS method. Results The RCF of kaempferide and isohamnetin with reference to quercetin were 0. 9499, 1. 0275. There is no significant difference between the calculated value of QAMS method and the measured value of external standard method. Conclusion With the quercetin as reference, simultaneous determination of quercetin, kaempferide, isohamnetin in Hippophae Rhamnoides L. by QAMS method is accurate and reliable, which can provide scientific basis for the quality control of the flavonoids in Hippophae Rhamnoides L. .关键词
一测多评/沙棘果/槲皮素/山柰素/异鼠李素/含量测定Key words
quantitative analysis of multi-components by single marker/Hippophae Rhamnoides L./quercetin/kaempferide/isohamnetin/content determination分类
医药卫生引用本文复制引用
刘峰,马久太,王浩仁,李瑾,党艳妮,李炜..一测多评法测定沙棘鲜果中槲皮素、山柰素和异鼠李素含量[J].中国药业,2017,26(13):24-27,4.基金项目
陕西省重点科技创新团队计划[ 2015KCT-19 ] （）
陕西省创新药物研究中心项目[ 2015SF2-09 ] （）
陕西省咸阳市重大科技专项计划项目[ 2014k01-18 ]. （）
