中国药业2017，Vol.26Issue(13)：28-30,3.DOI:10.3969/j.issn.1006-4931.2017.13.008
动态浊度法测定丙泊酚中细菌内毒素含量
Content Determination of Bacterial Endotoxin in Propofol by Kinetic Turbidimetric Assay
杨柳1
作者信息
- 1. 重庆市食品药品检验检测研究院·重庆市药物过程与质量控制工程技术研究中心,重庆 401121
- 折叠
摘要
Abstract
Objective To establish a kinetic turbidimetric assay ( KTA ) method for determine the concentration of bacterial endotoxin in propofol. Methods Bacterial endotoxin was added into test sample for interference test, and the recovery rate was determined, no inter-ference concentration was determined for the sample. The linear range and bacterial endotoxin in samples were determined. Results The linear range of bacterial endotoxin was 0. 03125-2. 0 EU/mL ( r= -0. 9954 ) . The sample had no interference to the test when it is diluted to 1. 25 g/L and below. The recovery rate of bacterial endotoxin was 50% -200%. The concentration of endotoxin in the 3 batches of samples met the requirements for bacterial endotoxins. Conclusion The KTA method is suitable for the content determination of bacterial endotoxin in propofol.关键词
丙泊酚/细菌内毒素/动态浊度法/含量Key words
propofol/bacterial endotoxin/kinetic turbidimetric assay/content分类
医药卫生引用本文复制引用
杨柳..动态浊度法测定丙泊酚中细菌内毒素含量[J].中国药业,2017,26(13):28-30,3.
