中国真菌学杂志2017，Vol.12Issue(3)：135-139,5.
不同免疫抑制剂对小鼠罹患侵袭性肺曲霉病的影响
Comparison of two immunosuppressive agents on the infection and inflammation of experimental invasive pulmonary aspergillosis mice models
摘要
Abstract
Objective To establish an experimental immunosuppressed mice model of invasive pulmonary aspergillosis (IPA),to evaluated various parameters of the innate immune response during the progression of IPA.Methods Immunosuppressed mice received the intratracheal administration of A.fumigatus conidia after two immunosuppressive treatments:a corticosteroid (group A) and a cyclophosphamidum agent (group B).We compared host responses 24 h,48 h and 72 h after infection in terms of survival,lung tissue histopathological analysis,fungal burden,pulmonary production of pro-and anti-inflammatory cytokines.Results The group A showed longer survival than group B did (5.33 vs 2.5 d,P ＜0.05).Group A showed severe bleeding and congestive,and lung tissue granuloma formation;while group B showed visible A.fumigatus hyphae,tissue necrosis 48 h after infection.Through CFUs and A.fumigatus 18sRNA detections,fugal burdens were significantly lower in lungs of corticosteroid-treated mice than those of cyclophos-phamidum-treated mice.This difference was also observed in livers and kidneys,indicating that the level of fungal dissemination was higher in group B than that of group A.In group B mice,the IL-10 concentration increased by 48 h after infection and peaked at 72 h (86 + 15 pg/mL).No TNF-α production was detected.In group A mice,both TNF-α and IL-10 were detected at very high concentrations at 48 h (3 120±393,434 ± 97 pg/mL).IL-1 α expression increased 24 h after infection in group A mice and continually increased within the 3 days.But in group B,IL-1 α concentrations maintained with low level.In both group A and B,the expressions of IL-1β increased dramatically after infection.Conclusion Establishing the IPA animal models of the mice by corticosteroid and chemotherapytreated,We found that IPA pathogenesis was difference between the two groups:the inflammation response of lung was severe in corticosteroid-treated mice than those in chemotherapy-treated mice,while chemotherapy-treated mice showing an adverse host response.关键词
侵袭性曲霉感染/动物模型/小鼠/免疫/致病机制Key words
invasive pulmonary aspergillosis/animal model/mice/immunology/pathology分类
医药卫生引用本文复制引用
陈玉宝,陈菲菲,邹春芳,邵宏涛..不同免疫抑制剂对小鼠罹患侵袭性肺曲霉病的影响[J].中国真菌学杂志,2017,12(3):135-139,5.基金项目
国家自然科学基金(81200004),南京市医学科技发展重点项目(ZKX13014) （81200004）
