中国真菌学杂志2017，Vol.12Issue(3)：144-147,4.
白念珠菌CaCSC1基因的敲除和功能研究
Disruption and Functions of CaCSC1 in Candida albicans
摘要
Abstract
Objective To construct deletion mutants for Candida albicans CaCSC1 and explore its functions.Methods The CaCSC1 knockout cassette containing the SAT1 flipper was PCR amplified using a pair of long primers.The cassette DNA was transformed into the wild type CAI4 strain,and genotypes of correct mutant strains were confirmed by PCR.Phenotypes of gene deletion mutants were screened by serial dilution assay.Results Gene deletion mutants of CaCSC1 were successfully constructed.Deletion mutants of (aCSC1 were sensitive to ketoconazole and tolerant against Zn2+,Mn2+ and Calcofluor white.Conclusions CaCsc1 participates in the regulation of zinc and magnesium homeostasis as well as plasma membrane and cell wall integrity in Candida albicans.关键词
白念珠菌/CaCSC1基因/基因敲除/离子稳态Key words
Candida albicans/CaCSC1/gene knockout/ion homeostasis分类
医药卫生引用本文复制引用
杨益,王亚楠,蒋伶活..白念珠菌CaCSC1基因的敲除和功能研究[J].中国真菌学杂志,2017,12(3):144-147,4.基金项目
国家自然科学基金(81371784,81571966) （81371784,81571966）
