中国肿瘤生物治疗杂志2017，Vol.24Issue(6)：615-619,5.DOI:10.3872/j.issn.1007-385X.2017.06.007
人参皂苷Rg3通过人乳腺珠蛋白A促进乳腺癌MDA-MB-231细胞凋亡及其可能的机制
Ginsenoside Rg3 promotes the apoptosis of breast cancer MDA-MB-231 cells via regulation of mammaglobin-A expression
摘要
Abstract
Objective:To study the mechanism of ginsenoside Rg3 inhibiting the proliferation of breast cancer MDA-MB-231 cells via regulation of mammaglobin-A (MGBA) expression.Methods:MTT assay and flow cytometry was used to detect the effect of different concentrations (5,10,15 μg/ml) of Rg3 on the proliferation and apoptosis of breast cancer cells;Western blotting was used to detect the expression of MGBA in breast cancer cells.MDA-MB-231 cells were treated with Rg3 and/or siRNA-MGBA;MTT and Flow cytometry were used to detect their effect on the proliferation and apoptosis of treated cells while Western blotting was used to detect the expression of MGBA in cells;sulfur electrode assay was used to detect H2S secretion in breast cancer MDA-MB-231 cells.Results:48 h after cell treatment,the inhibitory rates on proliferation of MDA-MB-231 cells in Rg3 groups (5,10,15 μg/ml) were significantly higher than that of control group ([18.78±0.82]％,[33.25±1.17]％,[35.11±0.94]％ vs [9.72-±0.91]％,allP＜0.05];Rg3 promoted the apoptosis of MDA-MB-231 cells (P ＜ 0.05),and significantly enhanced the expression of MGBA protein in MDA-MB-231 cells (P ＜0.05);Compared with the control group,the inhibitory rate on proliferation of MDA-MB-231 cells in Rg3 group was significantly increased ([30.12 ± 1.01) ％ vs (10.66 ± 0.59) ％,P ＜ 0.05],while the inhibitory rates in Rg3 + siRNA-MGBA and siRNA-MGBA group were significantly reduced ([6.61 ± 0.63] ％,[7.02 ± 0.46] ％ vs [10.66 ± 0.59] ％,all P ＜ 0.05);The expression of MGBA and cystathionine-γ-lyase (CSE) as well as the secretion ofH2S in Rg3 group were significantly enhanced;however,their expressions in Rg3 + siRNA-MGBA group and siRNA-MGBA group were significantly inhibited (all P ＜ 0.05).Conclusion:Rg3 can significantly inhibit the growth of MDA-MB-231 breast cancer cells and promote its apoptosis,and this effect may be realized by enhancing MGBA expression and activating H2S/CSE system.关键词
人参皂苷Rg3/人乳腺珠蛋白/乳腺癌/MDA-MB-231细胞Key words
ginsenoside Rg3/mammaglobin-A (MGBA)/breast cancer/MDA-MB-231 cell分类
医药卫生引用本文复制引用
孙大鹏,顾立学,李晨光,张凤香..人参皂苷Rg3通过人乳腺珠蛋白A促进乳腺癌MDA-MB-231细胞凋亡及其可能的机制[J].中国肿瘤生物治疗杂志,2017,24(6):615-619,5.基金项目
辽宁省自然科学基金资助项目(No.2014022044).Project support by Natural Science Foundation of Liaoning Province(No.2014022044) （No.2014022044）
