中国肿瘤生物治疗杂志2017，Vol.24Issue(6)：620-626,7.DOI:10.3872/j.issn.1007-385X.2017.06.008
褪黑素通过促凋亡协同增强顺铂对人喉癌细胞增殖的抑制作用
Melatonin synergistically enhances cisplatin-induced cell proliferation suppression of Hep-2 human laryngeal cancer ceils by promoting apoptosis
摘要
Abstract
Objective:Toinvestigate the effect of melatonin (MT) on humanlaryngeal cancercells and its ability to enhance the sensitivity of humanlaryngeal cancercells tocisplatin (DDP) treatment,this study was performed.Methods:Hep-2 human laryngeal cancer cells were treated with various concentrations of MT and/or DDP in vitro for various times.Then cells proliferation was detected by CCK-8 assays and cell apoptosis or cell cycle was assayed by flow cytometry.The co-efficient of drug interaction (CDI) was used to evaluate whether MT could affect the sensitivity of Hep-2 cells to DDP.Results:It was demonstrated by CCK-8 assays that MT or DDP used alone inhibited the proliferation of Hep-2 cells in a dose-dependent manner.Combined treatment with MT and DDP synergistically inhibited the proliferation of Hep-2 cells with the synergism between two drugs (CDI ＜ 1).Furthermore,flow cytometry results showed that MT promoted apoptotic cells and the proportion of cells in sub-G1 phase in Hep-2 cells,and co-treatment with MT and DDP increased apoptotic cells (the apoptotic rate of cells in the 0.5 mmol/L MT and 20 μg/ml DDP combination group was significantly higher than that in 20 μg/ml DDP group,[40.9 ± 3.0] ％ vs [11.0 ± 0.9] ％,P ＜ 0.01) and the proportion of cells in sub-G1 phase (the proportion of cells in sub-G1 phase in the 0.5 mmol/L MT and 20 μg/ml DDP combination group was significantly higher than that in 20 μg/ml DDP group,[73.0 ±2.4]％ vs [40.4 ±3.0]％,P＜0.01).Caspase inhibitor ZVAD-fmk reversed the suppression of Hep-2 cell proliferation and the induction of Hep-2 cell apoptosis by MT and/or DDP (both P ＜ 0.01).Conclusions:Our findings demonstrate that melatonin can induce Hep-2 human laryngeal cancer cell apoptosis in caspase-dependent manners,thereby synergistically enhancing the suppressive effects of cisplatin on human laryngealcancercellproliferation.关键词
褪黑素/顺铂/人喉癌/细胞凋亡/细胞周期Key words
melatonin/cisplatin/human laryngeal cancer/cell apoptosis/cell cycle分类
医药卫生引用本文复制引用
董龙宝,薛洁,黄安乐,张晶..褪黑素通过促凋亡协同增强顺铂对人喉癌细胞增殖的抑制作用[J].中国肿瘤生物治疗杂志,2017,24(6):620-626,7.基金项目
国家自然科学基金资助项目(No.81502466) （No.81502466）
南京军区医药卫生科研基金资助项目(No.14MS023).Projects supported by the National Natural Science Foundation of China (No.81502466),and the Medical and Health Research Foundation of Nanjing Military Area Command (No.14MS023) （No.14MS023）
