中国肿瘤生物治疗杂志2017，Vol.24Issue(6)：627-631,5.DOI:10.3872/j.issn.1007-385X.2017.06.009
5-Aza-CdR通过DNMT1调控卵巢癌细胞ERCC1基因甲基化及其表达
5-Aza-CdR effects the methylation and expression of ERCC1 gene in ovarian cancer cell via DNMT1
陈慧雁 1李晓翠 2沈宗姬2
作者信息
- 1. 苏州大学附属第一医院妇产科,江苏苏州215006
- 2. 同济大学附属上海市第一妇婴保健院妇产科,上海201204
- 折叠
摘要
Abstract
Objective:Objective:To investigate the effects of 5-Aza-CdR on expression of excision repair cross complementation group 1 (ERCC1) in ovarian cancer cell line SKOV3 and its possible mechanism.Methods:shRNA that specifically targeting DNA methyltransferase 1 (DNMT1) was constructed and transfected into SKOV3 cells.Western blotting was applied to determine the expressions of DNMT1 and ERCC1 in SKOV3 cells;Ovarian cancer cell line SKOV3 was treated with 5-Aza-CdR of various concentrations for different time courses,and Western blotting was used to determine the protein changes of DNMT1 and ERCC1 before and after the treatment;and the methylation level of ERCC1 promoter was tested by Sodium Bisulfite method.Results:After being treated with 5-Aza-CdR (at the concentrations of 0.5,1.0,2.0,4.0 μmol/L),the expression of DNMT1 protein in SKOV3 cells was decreased in a dose-dependent manner while the ERCC1 protein was increased in a time-dependent manner;With the treatment of 5-Aza-CdR (concentration of 1.0 μmol/L) for 12,24 and 36 h,the expression of DNMT1 protein was decreased and the ERCC1 protein was increased in SKOV3 cells,and the expression both changed in a time-dependent manner.Sodium Bisulfite method indicated that the methylation of ERCC1 promoter was at high level before the treatment;However,after the treatment of 5-Aza-CdR at concentration of 1.0 μmoL/L,the promoter of ERCC1 showed demethylation.Conclusion:5-Aza-CdR regulates the methylation of ERCC1 promoter and its expression in SKOV3 cells via.关键词
卵巢癌/甲基化/DNA甲基转移酶1基因/切除交叉修复互补基因1Key words
ovarian cancer/methylation/DNA methyltransferase 1 (DNMT1)/excision repair cross complementation group 1 (ERCC1)分类
医药卫生引用本文复制引用
陈慧雁,李晓翠,沈宗姬..5-Aza-CdR通过DNMT1调控卵巢癌细胞ERCC1基因甲基化及其表达[J].中国肿瘤生物治疗杂志,2017,24(6):627-631,5.
