中国肿瘤生物治疗杂志2017，Vol.24Issue(6)：632-638,7.DOI:10.3872/j.issn.1007-385X.2017.06.010
非小细胞肺癌组织中黑色素瘤相关抗原A家族部分成员的表达及其意义
Expressions of the melanoma antigen-A family member in non-small cell carcinoma tissues and its significance
摘要
Abstract
Objective:To investigate the expression of melanoma antigen (MAGE)-As in non-small cell carcinoma (NSCLC),and to explore its correlation with clinical pathological indicators and the prognosis.Methods:NSCLC tissue samples (n =90) and para-carcinoma tissue samples (n =90) were collected from 90 patients with NSCLC who were surgically treated in Fourth Hospital of Hebei Medical University between September of 2005 and March of 2006.The expression of MAGE-As protein in the 90 paired tissues was detected by immunohistochemistry assay,and its correlation to NSCLC clinical pathological indicators was analyzed.In addition,EGFR amplification and ALK rearrangements of NSCLC patients were detected by fluorescent in situ hybridization,and the association between MAGE-As expression and EGFR amplification and ALK rearrangements was analyzed with Spearman correlation analysis.The survival of NSCLC patients with positive MAGE-As expression was analyzed by Kaplan-Meier method.Cox regression model was used for univariate analysis and multivariate analysis of correlations between positive expression of MAGE-As and clinical pathological indicators.Results:The positive rates of MAGE-As protein in NSCLC were significantly higher than that of non-cancerous tissues (P ＜ 0.05).MAGE-As expression was not correlated with clinical pathological indicators of NSCLC patients,EGFR amplification and ALK rearrangements (P ＞ 0.05).Log-rank test showed that the survival time of NSCLC patients with positive MAGE-As expression were lower than those with negative expression (P ＜ 0.05);However,EGFR amplification and ALK rearrangements were not associated with the overall survival of NSCLC patients.Results from multivariate analyses showed that the expression of MAGE-As,clinical stage,lymph node metastasis were independent risk factors of NSCLC.Conclusion:MAGE-As proteins are NSCLC-associated antigens,thus having potential diagnostic and prognostic significance in clinical settings.关键词
非小细胞肺癌/黑色素瘤相关抗原-As/预后/EGFR扩增/ALK重排Key words
non-small cell carcinoma (NSCLC)/melanoma antigen-As/prognosis/EGFR amplification/ALK rearrangements分类
医药卫生引用本文复制引用
谷丽娜,桑梅香,尹丹静,刘飞,刘世娜,黄卫娜,王芃堉,连易水,单保恩..非小细胞肺癌组织中黑色素瘤相关抗原A家族部分成员的表达及其意义[J].中国肿瘤生物治疗杂志,2017,24(6):632-638,7.基金项目
河北省财政支撑资助项目(No.20141257) （No.20141257）
河北省杰出青年基金资助项目(No.H2016206410) （No.H2016206410）
河北省财政厅资助项目(No.2016361006). Project supported by the Financial Supporting Program of Hebei Province(No.20141257),the Foundation for Distinguished Young Scholars of Hebei Province(No.H2016206410),and the Financial Department of Hebei Province (No.2016361006) （No.2016361006）
