中国中医药信息杂志2017，Vol.24Issue(8)：54-59,6.DOI:10.3969/j.issn.1005-5304.2017.08.013
加味当归贝母苦参丸对MFC胃癌荷瘤小鼠瘤组织Toll样受体通路分子的影响
Effects of Modified Danggui Beimu Kushen Pills on Toll-like Receptor Pathway Markers on MFC Gastric Cancer Mice
摘要
Abstract
Objective To study the effects of modified Danggui Beimu Kushen Pills on the expressions of TLR2, TLR4, TLR6, TRAF6 and MyD88 in tumor tissues on MFC gastric cancer bearing mice; To discuss relevant mechanism of action. Methods MFC gastric cancer bearing mice were employed to perform anti-tumor experiment in vivo in this study. A total of eligible 48 mice were randomly divided into model group, DDP positive control group, modified Danggui Beimu Kushen Pills high-dose and low-dose groups, modified Danggui Beimu Kushen Pills high-dose and low-dose combined with DDP groups. The treatment was conducted once a day, and lasted for 14 continuous days. After the last administration of gavage orally treatment, all mice were anaesthetized and killed by cervical dislocation method to obtain tumor tissue completely for further HE staining measure and detection of TLR2, TLR4, TLR6, TRAF6 and MyD88 in tumor tissue with the method of RT-qPCR and immunohistochemistry. Meanwhile, the tumor growth was observed and the general conditions of mice were recorded. Results The model group was rich in tumor cells; the sizes of cells were different; the volume was large; the nucleus was deeply stained and the heterotypic shape was obvious, and the small focal necrosis was seen. The number of tumor cells in each administration group was reduced; the arrangement was loose; the cell volume was significantly reduced, and the nuclear pyknosis was reduced. Cell necrosis significantly increased; the number of interstitial blood vessels decreased; collagen fibers increased, especially in modified Danggui Beimu Kushen Pills high-dose and low-dose combined with DDP groups. Compared with the model group, the expressions of TLR2, TLR4, TLR6, TRAF6 and MyD88 mRNA and protein decreased in each administration group. TLR2, TLR4, TLR6, TRAF6 and MyD88 were lighter and weakly positive expressed in modified Danggui Beimu Kushen Pillshigh-dose and low-dose combined with DDP groups, the protein changes were more obvious Compared with DDP positive control group, modified Danggui Beimu Kushen Pills high-dose and low-dose groups. Conclusion Modified Danggui Beimu Kushen Pills can down-regulate TLR2, TLR4, TLR6, TRAF6 and MyD88 expression of tumor tissue in MFC gastric cancer bearing mice at both mRNA and protein levels to play anti-tumor pharmacology action.关键词
加味当归贝母苦参丸/胃癌/Toll样受体通路/小鼠Key words
modified Danggui Beimu Kushen Pills/gastric cancer/Toll-like receptor pathway/mice分类
医药卫生引用本文复制引用
李海龙,王勇,翟宇,程小丽,安霞,李红亮,康万荣,吴红彦..加味当归贝母苦参丸对MFC胃癌荷瘤小鼠瘤组织Toll样受体通路分子的影响[J].中国中医药信息杂志,2017,24(8):54-59,6.基金项目
甘肃省科技厅自然科学基金B类计划项目(1310RJZA099) （1310RJZA099）
甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2015-010) （ZDSYS-KJ-2015-010）
