中国卒中杂志2017，Vol.12Issue(7)：604-608,5.DOI:10.3969/j.issn.1673-5765.2017.07.008
腺苷酸活化蛋白激酶在星形胶质细胞中的表达
Expression of Adenosine Monophosphate-activated Protein Kinase in Cultured Rat Astrocytes
摘要
Abstract
Objective To explore the way of adenosine monophosphate-activated protein kinase (AMPK) forming into heterotrimer in astrocytes through detecting the expression of AMPK α1, α2, β1, β2, γ1 and γ2 subunits in cultured rat astrocytes. Methods Primary cultured astrocytes derived from the cerebral cortex of one-day-old Wistar rats. The purity of cell culture was assessed by immunofluorescence staining for anti-glial fibrillary acidic protein (GFAP) and S100β antibody. The expression of AMPK subunits were detected through reverse transcription polymerase chain reaction (RT-PCR) in cultured astrocytes. Results Immunofluorescence staining revealed that over 95% of cells were GFAP and S100β-positive, and the monolayer cultures of astrocytes were presented as uniform shapes under the phase contrast microscopic. The results from RT-PCR showed that the five subunits including AMPK α1, α2,β1, γ1 and γ2 were found in the cultured astrocytes. The expression of AMPK α2, β1 and γ1 subunits were relatively high, and AMPK α1 and γ2 subunit expression were very weak. Conclusion AMPK heterotrimer is composed of α2, β1 and γ1 subunits in astrocytes, and plays a key role in the regulation of energy metabolism in astrocytes.关键词
腺苷酸活化蛋白激酶/星形胶质细胞/细胞培养/缺血性脑损伤Key words
Adenosine monophosphate-activated protein kinase/Astrocyte/Cell culture/Ischemic brain injury引用本文复制引用
师忠芳,徐立新,闫旭,董丽萍,袁芳..腺苷酸活化蛋白激酶在星形胶质细胞中的表达[J].中国卒中杂志,2017,12(7):604-608,5.基金项目
北京市自然科学基金项目(7152027)国家自然科学基金项目(81271286) （7152027）
