中成药2017，Vol.39Issue(7)：1398-1402,5.DOI:10.3969/j.issn.1001-1528.2017.07.015
UPLC-MS/MS法同时测定贞芪扶正胶囊中10种成分
Simultaneous determination of ten constituents in Zhenqi Fuzheng Capsules by UPLC-MS/MS
摘要
Abstract
AIM To develop a UPLC-MS/MS method for the simultaneous content determination of adenosine,salidroside,chlorogenic acid,calycosin-7-O-β-D-glycoside,specnuezhenide,calycosin,formononetin,quercetin,apigenin and astragaloside Ⅳ in Zhenqi Fuzheng Capsules (Ligustri lucidi Fructus and Astragali Radix).METHODS The analysis of methanol extract of this drug was performed on a 40 ℃ thermostatic Inertsutain C18 column (75 mm × 3.0 mm,2 μm),with the mobile phase comprising of methanol-acetonitrile-4 mmol/L ammonium acetate flowing at 0.5 mL/min in a gradient elution manner.RESULTS Ten constituents showed good linear relationships within their own ranges (r≥0.996 0),whose average recoveries were 95.1％-104.3％ with the RSDs of less than 4.20％.CONCLUSION This sensitive and specific method can be used for the rapid quality control of Zhenqi Fuzheng Capsules.关键词
贞芪扶正胶囊/化学成分/UPLC-MS/MSKey words
Zhenqi Fuzheng Capsules/chemical constituents/UPLC-MS/MS分类
医药卫生引用本文复制引用
房桂珍,刘笑,沈云云,莫海莹,刘保霞,李瑞宏,祁金龙..UPLC-MS/MS法同时测定贞芪扶正胶囊中10种成分[J].中成药,2017,39(7):1398-1402,5.基金项目
国家自然科学基金资助项目(21402139) （21402139）
河北省教育厅优秀青年基金资助项目(YQ2013033) （YQ2013033）
河北省医学科学研究重点课题计划项目(20150560) （20150560）
河北省食品药品监督管理局(重点)科技项目(ZD2015023) （重点）
