广东海洋大学学报2017，Vol.37Issue(4)：1-7,7.DOI:10.3969/j.issn.1673-9159.2017.04.001
马氏珠母贝PmLec-8基因的克隆与表达分析
Clone and Expression Analysis of PmLec-8 Gene from Pinctada fucata martensii
摘要
Abstract
Lec-8 (C-type lectin 8) belongs to the family of C-type lectin and plays an important role in resisting microorganism infection. A full length of PmLec-8 was obtained using rapid amplification of cDNA ends (RACE) technology from Pinctada fucata martensii. The expression patterns of PmLec-8 in all tissues were further tested by Quantitative Real-Time PCR technology. Results showed that the total length of PmLec-8 cDNA was 737 bp, including a 5′ UTR of 36 bp, a 3′ UTR of 113 bp and an open reading frame (ORF) of 588 bp which encodes 195 amino acids. The predicted molecular mass was 22.90 ku, and isoelectric point was 5.17. The protein encoded by PmLec-8 has a carbonhydrate-recognition domain (CRD), conforming to the characteristics of C-type lectin family. Multiple sequence alignment indicated that Lec-8 was less conservative among species with four conserved cysteine residues. qRT-PCR data revealed that PmLec-8 was expressed in all tested tissues, including hepatopancreas,gonads,adductor muscle,gill,hemocytes and mantle,with the highest expression in hepatopancreas,higher in gill (P<0.05). These results indicated that PmLec-8 may play an important role in resisting microorganism infection of Pinctada fucata martensii.关键词
马氏珠母贝,C-型凝集素,基因克隆,实时荧光定量/PCRKey words
Pinctada fucata martensii/C-type lectin/gene cloning/real-time PCR分类
农业科技引用本文复制引用
吴羽媛,郭志颖,梁海鹰,王庆恒,邓岳文,杜晓东..马氏珠母贝PmLec-8基因的克隆与表达分析[J].广东海洋大学学报,2017,37(4):1-7,7.基金项目
国家自然科学基金(31472306) （31472306）
广东省科技计划项目(2012A031100010) （2012A031100010）
广东省海港建设与渔业产业发展专项(A201608B15) （A201608B15）
国家级大学生创新创业训练项目(201410566005) （201410566005）
广东海洋大学大学生创新创业训练项目(CXXL2014012) （CXXL2014012）
