肿瘤防治研究2017，Vol.44Issue(6)：392-397,6.DOI:10.3971/j.issn.1000-8578.2017.16.1533
IL-4/IL-4R对肝细胞癌生物学行为的影响及潜在的作用机制
Effect of IL-4/IL-4R on Biological Behavior of Hepatocellular Carcinoma and Its Underlying Mechanism
摘要
Abstract
Objective To investigate the effect of Interleukin-4/Interleukin-4 receptor (IL-4/IL-4R) on the biological behavior of hepatocellular carcinoma(HCC) and its underlying mechanism.Methods Huh7 cells were cultured in vitro and then transfected with small interfering RNAs (siRNAs).Cell proliferation was assessed by CCK8.Cell cycle and apoptosis were analyzed by flow cytometry.The possible signaling proteins were measured by Western blot.Results The proliferation of Huh7 cells was increased by IL-4 (P=0.01),however the proliferation was suppressed by silencing IL-4R(P=0.00033).After knocking down IL-4R,the early apoptosis of Huh7 cells(P=0.014) and the expression of apoptosis-related proteins Bax(P=0.016),Caspase-3(P=0.029) were enhanced,and the expression of anti-apoptosis-related protein Bcl-2 was decreased(P=0.003).However the late apoptosis rate(P=0.108) and the cell cycle(G0/G1 phase,P=0.677;S phase,P=0.139;G2/M phase,P=0.855) were not changed.Furthermore,IL-4 up-regulated the phosphorylation of JAK1(P=0.01) and STAT6(P=0.005).The phosphorylation levels of JAK1(P=0.016) and STAT6(P=0.019)in Huh7 cells were down-regulated by IL-4R depletion.Conclusion IL-4/IL-4R can activate JAK1/STAT6 signaling pathway and enhance the proliferation of HCC.Furthermore,IL-4/IL-4R can inhibit the apoptosis of HCC through regulating the expression of apoptotic proteins and anti-apoptotic proteins.关键词
肝细胞癌/白细胞介素-4/白细胞介素-4受体/增殖/凋亡/细胞周期Key words
Hepatocellular carcinoma/Interleukin-4/Interleukin-4 receptor/Proliferation/Apoptosis/Cell cycle分类
医药卫生引用本文复制引用
国畅廓,蔡婧,熊乐,刘安文..IL-4/IL-4R对肝细胞癌生物学行为的影响及潜在的作用机制[J].肿瘤防治研究,2017,44(6):392-397,6.基金项目
江西省教育厅项目(GJJ14068) （GJJ14068）
