作物学报2017，Vol.43Issue(7)：967-973,7.DOI:10.3724/SP.J.1006.2017.00967
十个八倍体小偃麦的细胞学鉴定和染色体构成分析
Cytological Identification and Chromosome Constitution Analyses of Ten Octo-ploid Trititrigia Accessions
摘要
Abstract
Developing new octoploid Trititrigia is of great significance for using Thinopyrum intermedium to the genetic im-provement of common wheat. Ten octoploid Trititrigia accessions, i.e., Shannong TE256, Shannong TE259, Shannong TE261, Shannong TE262, Shannong TE263, Shannong TE265, Shannong TE266, Shannong TE267-1, Shannong TE270, and Shannong TE274, were developed from the progenies of the cross between Thinopyrum intermedium and common wheat variety 'Yannong 15'. In this study, cytological methods and genomic in situ hybridization (GISH) were employed to determine cytological stability and chromosome constitutions of the 10 octoploid Trititrigia accessions. Mitotic observation indicated that most plants of octo-ploid Trititrigia had 56 chromosomes and a few had 54 or 55 chromosomes. Chromosomes in most pollen mother cells of plants with 2n = 56 formed 28 bivalents, showing a high degree of cytogenetic stability, simultaneously, univalents, trivalents and tetra-valents appeared occasionally at meiotic metaphase Ⅰ. At meiosis anaphase Ⅰ (PMC AI), most chromosomes segregated equally to the two poles except for several univalent chromosomes moving ahead in very few cells. Fourteen Th. intermedium chromosomes were observed to be added to the whole set of common wheat chromosomes in each octoploid Trititrigia, and the alien chromo-some constitutions of the 10 octoploid Trititrigia accessions were 2St+8JS+2J+2J-St, 2St+8JS+4J, 2St+8JS+2J+2J-St, 2St+8JS+2J+2J-St, 2St+8JS+2J+2J-St, 6St+4JS+2J+2J-St, 4St+6JS+2J+2J-St, 2St+8JS+4J, 2St+8JS+4J, and 4St+6JS+4J. These alien chromosome constitutions were different from those reported in octoploid Trititrigia, suggesting that the 10 octoploid Trititrigia accessions are novel materials and might be valuable in wheat breeding programs.关键词
中间偃麦草/八倍体小偃麦/GISH/染色体构成Key words
Th. intermedium/Octoploid Trititrigia/GISH/Chromosome constitution引用本文复制引用
亓晓蕾,鲍印广,李兴锋,钱兆国,王瑞霞,吴科,王洪刚..十个八倍体小偃麦的细胞学鉴定和染色体构成分析[J].作物学报,2017,43(7):967-973,7.基金项目
本研究由国家重点研发计划项目(2016YFD0102004)和山东省现代农业产业技术体系项目(SDAIT-04-021-12)资助.This study was supported by the Key Project of the National Research and Development Program (2016YFD0102004) and the Modern Agri-cultural Technology System of Shandong Province (SDAIT-04-021-12). （2016YFD0102004）
