作物学报2017，Vol.43Issue(7)：974-982,9.DOI:10.3724/SP.J.1006.2017.00974
水稻紫鞘染色体片段代换系Z519的鉴定及PSH1候选基因分析
Identification of Rice Chromosome Segment Substitution Line Z519 with Pur-ple Sheath and Candidate Gene Analysis of PSH1
摘要
Abstract
Anthocyanins as plant pigments are widely liked by people and play a very important role in food processing and hy-brid purity identification. Here, a rice chromosome segment substitution line (CSSL) Z519 with purple sheath was identified de-riving from recipient Nipponbare and donor R225. Z519 contained 16 substitution segments with 6.85 Mb of average length, which were distributed on 11 chromosomes of rice except the 10th chromosome. The bud sheath of Z519 began to appear the purple color stripes when it was about 3 mm long. Then the purple stripes displayed on sheaths, leaf margins, vascular bundles of stem and stigmas. While all parts of Nipponbare were green. Anthocyanin content in leaf sheath of Z519 was significantly higher than that of Nipponbare, whereas no significant difference was in flag leaf. Compared with Nipponbare, plant height of Z519 was significantly decreased, spikelets number and grain number of main panicle, and 1000-grain weight of Z519 were significantly increased. There was no significant difference between Z519 and Nipponbare in the other traits such as panicle number, main panicle length and seed-setting rate. Then, F2 population from the cross of Nipponbare and Z519 were used for genetic analysis and gene mapping of the purple sheath. The purple sheath in Z519 was controlled by a single dominant gene, named as PSH1, which was mapped on the chromosome 1 between InDel marker L03 and SSR marker L01 with the physical distance of 37.8 kb. By sequencing and gene-predicting in the region, Z519 had three bases (GTG) insertion in the GTG repeat area of the 238th-252th base in the first exon compared with Nipponbare, which resulted in increasing a Gly amino acid. Furthermore, the expression of LOC_Os01g45910 was obviously decreased in Z519 by qRT-PCR analysis. Thus, LOC_Os01g45910 was prelimi-nary identified as the candidate gene of PSH1. The results lay a good foundation for studying molecular mechanisms of regulating anthocyanin by PSH1.关键词
水稻/染色体片段代换系/紫鞘PSH1/基因定位Key words
Rice (Oryza sativa L.)/Chromosome segment substitution line (CSSL)/Purple sheath PSH1/Gene mapping引用本文复制引用
周可,李燕,王世明,崔国庆,杨正林,何光华,凌英华,赵芳明..水稻紫鞘染色体片段代换系Z519的鉴定及PSH1候选基因分析[J].作物学报,2017,43(7):974-982,9.基金项目
本研究由国家重点研发计划项目(SQ2016ZY03001818)的"水稻杂种优势利用创新技术研究"课题(2016YFD0101107)和重庆市重点实验室能力提升项目(cstc2014pt-sy80001)资助.This study was supported by the subject "New Technology of Heterosis Utilization in Rice" (2016YFD0101107) in the National Key Research and Development Program (SQ2016ZY03001818), and Chongqing Key Laboratory Capacity Improvement Project (cstc2014pt-sy80001). （SQ2016ZY03001818）
