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耐低氮烟草基因型的筛选及其氮效率类型

钟思荣 陈仁霄 陶瑶 龚丝雨 何宽信 张启明 张世川 刘齐元

作物学报2017,Vol.43Issue(7):993-1002,10.
作物学报2017,Vol.43Issue(7):993-1002,10.DOI:10.3724/SP.J.1006.2017.00993

耐低氮烟草基因型的筛选及其氮效率类型

Screening of Tobacco Genotypes Tolerant to Low-Nitrogen and Their Nitrogen Efficiency Types

钟思荣 1陈仁霄 2陶瑶 1龚丝雨 1何宽信 2张启明 2张世川 1刘齐元1

作者信息

  • 1. 江西农业大学农学院 / 作物生理生态与遗传育种教育部重点实验室 / 江西省作物生理生态与遗传育种重点实验室, 江西南昌330045
  • 2. 江西省烟叶科学研究所, 江西南昌 330045
  • 折叠

摘要

Abstract

Genotypes with tolerance to low nitrogen (N) are preferred in improving N use efficiency of tobacco and reduce nitro-gen pollution. In this study, seedlings of 74 tobacco genotypes were evaluated in a hydroponic experiment with low-N (0.5 mmol L–1) and normal-N (5.0 mmol L–1) treatments, and the genotypes were evaluated according to descriptive statistics, factor analysis and cluster analysis. The root volume, root biomass, N accumulation in stalk and leaf, and aboveground biomass varied greatly across genotypes with the variation coefficients of 0.37–0.68 in low-N treatment and 0.38–0.64 in normal-N treatment. The prin-cipal components under both N levels were similar, showing that the N accumulation in stalk and leaf and aboveground biomass were important components. According to evaluation indexes Heatmap clustering and scatter diagram analysis of N efficiency comprehensive value, 15 low-N tolerant genotypes were screened out, accounting for 20.3% of varieties tested, including eight genotypes of high-efficiency under low-N but low-efficiency under normal-N, six genotypes of low-efficiency under both low-N and normal-N, and one genotype of high-efficiency under both low-N and normal-N. In addition, eight genotypes were found to be sensitive to low-N, including six genotypes of low-efficiency under low-N but high-efficiency under normal-N and two geno-types of low-efficiency under both low-N and normal-N. We primarily identified 14P9 as the low-N tolerant and N-efficient to-bacco and Zhongyan 100 and K394 as the low-N sensitive and N-inefficient tobacco.

关键词

烟草/基因型/苗期/氮素/氮高效

Key words

Tobacco/Genotype/Seedling stage/Nitrogen/High nitrogen efficiency

引用本文复制引用

钟思荣,陈仁霄,陶瑶,龚丝雨,何宽信,张启明,张世川,刘齐元..耐低氮烟草基因型的筛选及其氮效率类型[J].作物学报,2017,43(7):993-1002,10.

基金项目

本研究由江西省烟草专卖局科技项目(201401001)资助.This study was supported by the Jiangxi Provincial Tobacco Monopoly Bureau of Science and Technology Project (201401001). (201401001)

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