作物学报2017，Vol.43Issue(7)：1003-1011,9.DOI:10.3724/SP.J.1006.2017.01003
白芝麻籽粒油脂、蛋白质及芝麻素含量QTL定位分析
QTL Mapping for Oil, Protein and Sesamin Contents in Seeds of White Sesame
摘要
Abstract
Seed oil content, protein content and sesamin content are three important targets for sesame breeding. The objective of this study was to explore the genetic model and detect quantitative trait loci (QTLs) for three quality traits using a mapping popu-lation containing 224 recombinant inbred lines (RILs, F9). The three quality traits of seeds were measured by near infrared reflec-tance spectroscopy in three environments. Correlation analysis showed oil content was negatively correlated with protein content but positively correlated with sesamin content, whereas, protein content was negatively correlated with sesamin content. Using mixed composite interval mapping (MCIM) method, eight QTLs for the three seed quality traits were detected with heritability of additive effect ranging from 0.41% to 14.55%. Using multiple interval mapping (MIM) method, 13 QTLs were mapped on nine linkage groups (LGs), and a single QTL explained 5.2%–18.6% of the total phenotypic variation. Among them, five loci had at least one marker identical to the QTL identified with MCIM, indicating they might be located in the same region. Two QTLs were detected in multiple environments. QTLs Qoc-5 and Qsc-5 were located in the same region on LG5, both had positive effects, whereas Qpc-5 in the adjacent region had a negative effect. Similar loci exist on LG2 and LG1, probably caused by pleiotropism or close linkage of several QTLs. These results suggest that the oil and sesamin contents in seed can be improved together, while the protein content needs to be selected negatively with the oil or sesamin content.关键词
芝麻/含油量/蛋白质含量/芝麻素含量/QTLKey words
Sesame/Oil content/Protein content/Sesamin content/QTL引用本文复制引用
吴坤,吴文雄,杨敏敏,刘红艳,郝国存,赵应忠..白芝麻籽粒油脂、蛋白质及芝麻素含量QTL定位分析[J].作物学报,2017,43(7):1003-1011,9.基金项目
本研究由国家自然科学基金项目(31201243), 国家重点基础研究发展计划(973计划)项目(2011CB109304)和国家现代农业产业技术体系建设专项(CARS-15)资助.The study was supported by the National Natural Science Foundation of China (31201243), the National Key Basic Research Program of China (973 Program) (2011CB109304), and the China Agriculture Research System (CARS-15). （31201243）
