作物学报2017，Vol.43Issue(7)：1012-1020,9.DOI:10.3724/SP.J.1006.2017.01012
茶树CsbZIP4转录因子正调控拟南芥对盐胁迫响应
Positive Regulation of CsbZIP4 Transcription Factor on Salt Stress Response in Transgenic Arabidopsis
摘要
Abstract
Basic region / leucine zipper (bZIP) transcription factors is a multi-functional protein family in eukaryotes, which is involved in various biological processes including seed maturation, light signaling regulation and stress response. According to sequence similarity and conserved motifs, the AtbZIPs are classified into 10 groups (A, B, C, D, E, F, G, H, I, and S). In this study, CsbZIP4 of C-group bZIP transcription factors was using as an objective, the expression patterns of CsbZIP4 in response to abiotic stress in tea plants were investigated, and the salinity tolerance caused by CsbZIP4 overexpression in Arabidopsis was analyzed. The CsbZIP4 from tea plant leaves was up-regulated under 4℃, exogenous ABA, salinity and dehydration stresses, especially the induction of salinity and dehydration stresses led to up-regulation of 2.9-fold and 2.2-fold, respectively. In roots, CsbZIP4 was down-regulated in response to cold, salinity and dehydration stresses, especially down-regulated by 2-fold under salinity stress. Under fluorescence microscope, it was indicated that CsbZIP4 was located in nucleus. Constitutive overexpression of CsbZIP4 in transgenic Arabidopsis lowered the plants' sensitivity to exogenous ABA and salinity at germination stage. More-over, CsbZIP4 overexpression lines exhibited higher salinity tolerance under 300 mmol L–1 NaCl conditions and higher SPAD values. AtSOS1, which is a salinity responsive gene, was strongly induced in overexpression lines. In conclusion, CsbZIP4 posi-tively regulates salt stress response in Arabidopsis, and might be closely related to the tolerance to salinity stress in tea plant.关键词
茶树/CsbZIP4转录因子/非生物胁迫/耐盐性Key words
Tea plant/CsbZIP4 transcription factor/Abiotic stress/Salinity tolerance引用本文复制引用
曹红利,王璐,钱文俊,郝心愿,杨亚军,王新超..茶树CsbZIP4转录因子正调控拟南芥对盐胁迫响应[J].作物学报,2017,43(7):1012-1020,9.基金项目
本研究由浙江省自然科学基金项目(LY14C160001), 国家自然科学基金项目(31500564), 国家现代农业产业技术体系建设专 项(CARS-23)和中国农业科学院农业科技创新工程项目(CAAS-ASTIP-2014-TRICAAS)资助.This study was supported by the Natural Science Foundation of Zhejiang Province (LY14C160001), the National Natural Science Foundation of China (31500564), the Earmarked Fund for China Agriculture Research System (CARS-23), and the Chinese Academy of Agricultural Sciences through an Innovation Project for Agricultural Sciences and Technology (CAAS-ASTIP-2014-TRICAAS). （LY14C160001）
