作物学报2017，Vol.43Issue(7)：1021-1029,9.DOI:10.3724/SP.J.1006.2017.01021
玉米热激转录因子基因ZmHsf25的克隆、特性与耐热性功能分析
Cloning, Characteristics and Regulating Role in Thermotolerance of Heat Shock Transcription Factor (ZmHsf25) in Zea mays L.
摘要
Abstract
Heat shock transcription factors (Hsfs) are key components of signal transduction pathways involved in the activation of genes in response to heat shock (HS) stress in plants. There are at least 30 Hsf members in maize and seven of which belong to class B. In our previous work, we obtained ZmHsf06, which belongs to subclass A1, and investigated the characteristics of expres-sion, subcellular localization, and regulating roles in thermotolerance and drought-stress tolerance of ZmHsf06. In the present study, ZmHsf25 was isolated from maize (Zea mays L.) young leaves treated with at 42℃ for one hour using homologous cloning methods. The sequencing analysis showed that the coding sequence (CDS) of ZmHsf25 was 957 bp and encoded a protein of 318 amino acids. The amino acid sequence analysis demonstrated that ZmHsf25 contained a DNA-binding domain (DBD), a nuclear localization signal (NLS) of KRLR peptide and a nuclear export signal (NES) of VLTLSV peptide. The identity of amino acid between ZmHsf25 and Sb060g025710 of sorghum was the highest, which was 92%. ZmHsf25 was expressed in multiple tissues and organs of maize, and transcription expression level of ZmHsf25 was the highest in pollens compared with root, stem, func-tional leaf, immature embryo and ear. qRT-PCR results showed that ZmHsf25 was up-regulated by 42℃ heat shock in both leaves and roots. Under normal conditions, ZmHsf25 was down-regulated by both salicylic acid CSA and H2O2, but significantly up-regulated by heat stress at 42℃. Through transient reporter assay with onion (Allium cepa L.) epidermal cells, we found that ZmHsf25 was localized in nuclei. ZmHsf25 overexpressed yeast showed stronger thermotolerance than the controls after heat shock (HS), though yeast thermotolerances were also decreased by HS. The results revealed that ZmHsf25 perhaps is one of downstream elements of SA signal pathway to play a key role in regulating the response to heat stress and pollen development. These results provide a theoretical basis for analyzing biological characteristics and functions of maize Hsf members further.关键词
玉米/热激转录因子基因ZmHsf25/表达/亚细胞定位/耐热性Key words
Maize/ZmHsf25/Expression/Subcelullar-localization/Thermotolerance引用本文复制引用
赵立娜,段硕楠,张华宁,郭秀林,李国良..玉米热激转录因子基因ZmHsf25的克隆、特性与耐热性功能分析[J].作物学报,2017,43(7):1021-1029,9.基金项目
本研究由河北省自然科学基金项目(C2017301065), 河北省博士基金项目(2017039349), 渤海粮仓科技工程项目(F16C14001)和河北省高层次人才项目(A201500130)资助.This study was supported by the Natural Science Foundation of Hebei Province (C2017301065), the Doctoral Foundation of Hebei Province (2017039349), the Bohai Barn Science and Technology Project (F16C14001) and the High-level Talent Project of Hebei Province (A201500130). （C2017301065）
