植物研究2017，Vol.37Issue(4)：613-620,8.DOI:10.7525/j.issn.1673-5102.2017.04.018
响应面优化长白落叶松体胚形成早期培养条件的研究
Response Surface Optimization of Culture Conditions for the Formation of Early Embryos in Larix olgensis
摘要
Abstract
With the larch embryogenic callus,through the Design Box-Behnken design test,we established a response surface model based on the somatic embryogenesis.The culture conditions of somatic embryo early maturation was optimized in Larix olgensis.The relative ion concentration (P =0.007),the inositol concentration(P =0.000),the culture period (P =0.000) and the interaction between inositol content and culture period (P =0.007) effect on the number of somatic embryos of L.olgensis were extremely significant.Cultivation of Larix somatic embryo maturation was 337.04 ind · g-1 at the early stage of the most optimum conditions for relative ion content of 26.767 7％ BM basic culture medium,inositol 10.454 5 g · L-1,culture time 12.656 6 d,and the number of somatic embryos average.In order to facilitate the practical operation,the culture condition was changed to the relative ion concentration of 25％ BM,inositol 10 g · L-1,incubation period of 13 d,then the average number of somatic embryos was 336.46 ind · g-1,that was consistent with the predicted value 336.29 ind · g-1.Compared with previous test,the quantity and quality of L.olgensis somatic embryos were increased significantly.关键词
落叶松属/长白落叶松/响应面优化/体胚发生/胚胎形成早期Key words
Larix/Larix olgensis/response surface optimization/somatic embryogenesis/early embryo formation分类
农业科技引用本文复制引用
宋跃,李淑娟,白晓明,张含国..响应面优化长白落叶松体胚形成早期培养条件的研究[J].植物研究,2017,37(4):613-620,8.基金项目
国家高技术研究发展计划(“863”计划2013AA102704-04-03)National High-tech R&D Program("863" Program 2013AA102704-04-03) （“863”计划2013AA102704-04-03）
