中药材2017，Vol.40Issue(2)：284-289,6.DOI:10.13863/j.issn1001-4454.2017.02.008
四倍体黄芩新品系D20和二倍体黄芩化学成分的比较研究
Comparative Study on Chemical Constituents Between Tetraploid and Diploid Scutellaria baicalensis
摘要
Abstract
Objective:To compare the chemical constituents between the tetraploid new strains D20 and the cultivated diploid Scutellaria baicalensis in Gansu province.Methods:Using ultra performance liquid chromatography(UPLC) to set up the fingerprint,and to simultaneously determine nine components in Scutellaria baicalensis.Results:The similarity index of new strain D20 tetraploid and diploid were greater than 0.99,The contents of baicalin,wogonoside,baicalein,wogonin,scutellarin,luteolin,apigenin,apigenin-7-O-β-D-glucuronide and oroxylin-A of diploid were (14.72 ± 0.46) ％,(5.84 ± 0.31) ％,(1.55 ± 0.49) ％,(0.72 ± 0.21) ％,(0.81 ± 0.04) ％,(1.47 ± 0.17) ％,(0.55 ± 0.01) ％,(0.30 ± 0.04) ％,(0.12 ± 0.01) ％,respectively,while in tetraploid the results were (16.54 ± 1.45)％,(6.62 ±0.39)％,(0.28 ±0.01)％,(0.20 ±0.02)％,(0.87 ±0.06)％,(1.50±0.19)％,(0.19 ±0.04)％,(0.31 ± 0.01)％,(0.10 ± 0.04)％,respectively.Conclusion:The results show that different ploidy have almost the same essential chemical constituent but the different relative contents.Except the baicalein,wogonin,oroxylin-A and apigenin of tetraploid were lower than those of diploid,the others five components of the tetraploid are higher than the diploid.关键词
黄芩/四倍体和二倍体/化学成分/UPLC指纹图谱Key words
Scutellaria baicalensis Georgi/Tetraploid and diploid/Chemical constituent/UPLC fingerPrint分类
医药卫生引用本文复制引用
刘玉,柴俊雯,张亚亚,王亚丽,郭玫,杜弢..四倍体黄芩新品系D20和二倍体黄芩化学成分的比较研究[J].中药材,2017,40(2):284-289,6.基金项目
甘肃省中药材产业科技攻关项目(GYC12-05) （GYC12-05）
中央本级重大增减支项目(2060302) （2060302）
