中药材2017，Vol.40Issue(2)：402-407,6.DOI:10.13863/j.issn1001-4454.2017.02.034
双丹脂质体凝胶剂的制备工艺优化及体外透皮性能研究
Research on Preparation Optimization and in vitro Percutaneous Permeability of Shuangdan Liposome Gels
摘要
Abstract
Objective:To optimize preparation process of Shuangdan liposome gels (SDLG),and to investigate its percutaneous permeability in vitro.Methods:Shuangdan liposome was prepared by the ethanol injection method.The rate of phosphatidyl choline (PC)-drug(X1),PC-cholesterol(X2),PC-octadecylamine (X3)and hydration temperature (X4)as independent variable.The encapsulation efficiency of tanshinol (Y1),panenol (Y2),and tanshinone Ⅱ A (Y3) as main evaluation indicator.The SDLG was optimized by using Box-Behnken response surface methodology and the characterization and in vitro transdermal was evaluated.Results:The optimal preparation process was as follows,X1 =13.82,X2 =4.57,X3 =13.28,X4 =50.10;The encapsulation efficiency of tanshinol was (30.62 ± 2.17)％,while panenol was (51.16 ± 2.54)％ and tanshinone was (80.47 ± 3.24)％.The average particle size of Shuangdan liposome was 240.8 nm,Zata potential was + 16.2 mV.Furthermore,the penetration rate of SDLG was lower than the conventional gel,gel residue is lower,but higher retain in dermis.Conclusion:Using Box-Behnken response surface methodology to optimize SDLG is completely doable.SDLG has obvious sustained-release effects,reduce side effects.关键词
双丹脂质体凝胶剂/Box-Behnken效应面法/包封率/体外透皮Key words
Shuangdan liposome gels/Box-Behnken response surface methodology/Encapsulation efficiency/In vitro percutaneous permeability分类
医药卫生引用本文复制引用
张小灵,郭嘉俊,时军,张慧迪,黄嗣航..双丹脂质体凝胶剂的制备工艺优化及体外透皮性能研究[J].中药材,2017,40(2):402-407,6.基金项目
广东省教育厅特色创新类项目(2015cxqx152) （2015cxqx152）
广东省科技计划项目(2013B021800088) （2013B021800088）
