中国现代中药2017，Vol.19Issue(6)：758-763,6.DOI:10.13313/j.issn.1673-4890.2017.6.005
新疆假龙胆的化学成分研究与含量测定
Study on Chemical Composition and Xanthones Content Determination of Gentianella turkestanorum (Gand.) Holub
摘要
Abstract
Objective:To preliminarily study on the chemical constituents of Gentianella turkestanorum (Gand.) Holub and analyze the content of xanthones by HPLC for quantitative analysis.Methods:The herbal material of G.turkestanorum was reflux-extracted by 75% ethanol,the crude extracts were fractionated with petroleum ether,ethyl acetate,and n-BuOH,respectively.The petroleum ethert and ethyl acetate part were extracted by silica gel column chromatography and Sephadex LH-20 column chromatography gradually to obtain the purified compounds,which were identified by spectral data EI-MS,1H-NMR and 13C-NMR.An HPLC method for the content determination of Xanthones compounds 1-3 was established.Results:Six compounds were isolated and identified from G.turkestanorum.,respectively,swerchirin (1),1-hydroxy-3,5,8-trimethoxyxanthone (2),1-hydroxy-3,5-demethoxyxanthone (3),luteolin (4),daucosterol (5) and β-sitosterol (6).The results showed that the conditions of the experiment exhibited better performance of separation for compounds 1-3.Compounds 1-3 formed a good linear relationship at 35.7~713.4,32~1280,34.3~686 ng,respectively.Precision RSD were 0.31%,0.27%,0.25% and the stability experiments of RSD were 1.05%,1.33%,1.58%,respectively.Repeated experiments RSD were 2.48%,0.82% and 2.63%.Meanwhile,the recovery rat were 103.1%(RSD=1.25%),100.02%(RSD=0.93%),95.10%(RSD=2.62%),respectively.The content of compounds 1-3 were 0.21%~0.28%,0.65%~0.77%,0.19%~0.15%,respectively.Conclusion:The chemical compositions of G.Turkestanorum were mainly flavonoids,xanthones and sterols.The established HPLC method is suitable for the determination of compounds 1-3 isolated from G.turkestanorum.Moreover,the contents of compounds 1-3 were shown in the higher level.关键词
新疆假龙胆/化学成分/黄酮/酮/含量测定Key words
Gentianella turkestanorum/chemical composition/flavonoids/xanthones/determination引用本文复制引用
邹德志,杨丹,王建华,韩华锐,白小荣,张春红,李旻辉..新疆假龙胆的化学成分研究与含量测定[J].中国现代中药,2017,19(6):758-763,6.基金项目
"十二五"国家科技支撑计划项目(2012BAI28B02) （2012BAI28B02）
国家自然基金项目(81060372) （81060372）
内蒙古自治区科技计划项目(蒙药资源保护与开发利用科研创新平台) （蒙药资源保护与开发利用科研创新平台）
