中国现代中药2017，Vol.19Issue(6)：771-774,778,5.DOI:10.13313/j.issn.1673-4890.2017.6.008
番茄红素对人肝癌HepG2细胞生长的影响及其机制
Effects of Lycopene on Growth of Human HepG2 Cell
摘要
Abstract
Objective:To investigate the effects of lycopene on the growth of human HepG2 cell.Methods:The human HepG2 cells in logarithmic growth phase was treated with lycopene (5,10,20 μg·mL-1) groups and cisplatin (40 μg·mL-1).The cellular growth inhibition rate was calculated by MTT,cell cycle and apoptosis rate were analyzed by flow cytometry,the expression of Bax,bcl-2 and cleaved caspase-3 protein was detected by western blotting.Results:The cellular growth inhibition rate of human HepG2 cell in lycopene treated groups and cisplatin treated group were significantly increased,the cell cycle was arrested at G0/G1 phase,the apoptosis rate was significantly increased,the expression of Bax mRNA was significantly increased,while the expression of bcl-2 was significantly decreased,and the ratio of Bax/bcl-2 was significantly increased;the expression of Cleaved Caspase-3 protein was significantly increased;all of the effects of Lycopene above were dose-dependent with a certain.Conclusion:Lycopene has inhibitive effects on the growth of human HepG2 cells perhaps through inhibiting proliferation and inducing apoptosis;which perhaps related to its effects of altering the cell cycle and the expression of apoptosis-related genes and proteins.关键词
番茄红素/肝癌/HepG2细胞/生长/抑制Key words
Lycopene/hepatic carcinoma/HepG2 cell/growth/inhibitive effects引用本文复制引用
赵孟雷..番茄红素对人肝癌HepG2细胞生长的影响及其机制[J].中国现代中药,2017,19(6):771-774,778,5.基金项目
邯郸市科学技术研究与发展计划项目(1623208086ZC) （1623208086ZC）
