中国现代中药2017，Vol.19Issue(6)：845-848,4.DOI:10.13313/j.issn.1673-4890.2017.6.021
正交设计优化拟缺香茶菜总黄酮提取工艺
Optimization of the Extraction Technology of Total Flavonoids fromIsodon excisoides by Orthogonal Experiment
摘要
Abstract
Objective:The optimal conditions of total flavonoids from Isodon excisoides were investigated by orthogonal array design.Methods:The content of total flavonoids was determined spectrometrically using rutin as the reference substance.The effects of extraction temperature,extraction time,liquid/material ratio,ethanol concentration on the content of total flavonoids in extracts were investigated by one-factor-at-a-time method,and the extraction conditions were optimized by orthogonal array design.Results:Ethanol concentration and liquid/material ratio were the key factors that affected the extraction of total flavonoids.The optimal extractive conditions were obtained as follows:temperature 90 ℃,treatment time 120 min,liquid/material ratio 50∶1 and ethanol concentration 50%.Under these conditions,the extraction yield of total flavonoids from I.excisoides was 5.668 6%.Conclusion:The optimized process remarkably increases the extraction yield of total flavonoids from I.excisoides and has a promising prospect for practical applications.关键词
拟缺香茶菜/总黄酮/正交试验/提取工艺Key words
Isodon excisoides/total flavonoids/orthogonal array design/extraction引用本文复制引用
陈慧平,李晓宁,罗含希,邹敏..正交设计优化拟缺香茶菜总黄酮提取工艺[J].中国现代中药,2017,19(6):845-848,4.基金项目
河南省自然科学基金(152300410159) （152300410159）
河南省高等学校重点科研项目(16A350011) （16A350011）
