郑州大学学报（理学版）2017，Vol.49Issue(3)：111-116,6.DOI:10.13705/j.issn.1671-6841.2017014
猪圆环病毒2型Cap蛋白B细胞表位的预测及鉴定
Prediction and Identification of B Cell Epitopes of the PCV2 Cap Protein
摘要
Abstract
Screening B cell epitopes of PCV2 Cap by bioinformatics techniques and prokaryotic expression systems,could provide a basis to further reveal the immune regulation mechanism of PCV2.The hydrophilicity,flexibility,accessibility,antigenicity and B-cell liner epitopes of PCV2 Cap were predicted by bioinformatics methods.There were 6 potential B-cell linear epitopes in Cap.They might locate in the regions of 58-66,79-91,151-162,174-189,204-213 and 221-233 aa.The potential epitopes gene were amplified by PCR using specific primers and cloned into the expression vector pET32a(+).E coli BL21(DE3)plysS cells were transformed with the recombinant plasmid;and the target proteins were expressed by induction using IPTG;B-cell linear epitopes of Cap protein were identified by detecting the reactivity of the target proteins with PCV2 positive serum.Three kinds of protein peptides (Cap77-95,Cap174-189,Cap221-233) with reactogenicity were selected.Analysis of homology indicated that three segments were unique to Cap which might be the specific epitopes of PCV2.关键词
猪圆环病毒2型/Cap蛋白/原核表达/表位Key words
porcine circovirus type 2/Cap protein/prokaryotic expression/epitopes分类
农业科技引用本文复制引用
周景明,宋瑞雪,刘红亮,张秋丽,祁艳华,张改平,王爱萍..猪圆环病毒2型Cap蛋白B细胞表位的预测及鉴定[J].郑州大学学报（理学版）,2017,49(3):111-116,6.基金项目
国家重点研发计划项目(2016YFD0500704) （2016YFD0500704）
郑州市科技创新团队项目(131PCXTD622). （131PCXTD622）
