安徽农业大学学报2017，Vol.44Issue(3)：367-372,6.DOI:10.13610/j.cnki.1672-352x.20170524.007
患体表出血症的泥鳅病原菌的鉴定与生物特性分析
Biological characterization and identification of bacteria from Misgurnus anguillicaudatus associated with body surface hemorrhagic disease
摘要
Abstract
Two bacteria associated with body surface hemorrhagic disease were isolated from the blood,heart,liver,spleen,intestines ofMisgurnus anguillicaudatus,which were further analyzed using morphological observation,physiological and biochemical characteristic analysis,16SrDNA sequence determination,sensitivity test and artificial infection experiments.The isolated strains were identified as Vibrio cholerae and Shewanella putrefaciens which were related to the pathogenicity of Misgurnus anguillicaudatus.The sensitivity of the two bacteria to 20 antibiotics was evaluated.The results showed that the isolated strains were highly sensitive to seven antibiotics including chloramphenicol,ceftazidime pentahydrate,norfloxacin,tetracycline,cefoxitin,paediatric compound sulfamethoxazole and ciprofloxacin.They also showed some resistance to other antibiotics.This is the first report on a new pathogen of Misgurnus anguillicaudatus.It revealed that Shewanella sp.,as a potential new pathogen,may have a synergistic effect with Vibrio cholerae and pose a threat to the culture ofMisgurnus anguillicaudatus.关键词
泥鳅/出血症/霍乱弧菌/希瓦氏茵/药敏试验Key words
Misgurnus anguillicaudatus/hemorrhagic disease/Vibrio cholerae/Shewanella putrefaciens/antibiotic susceptibility testing分类
农业科技引用本文复制引用
林锋,刘莉,包成荣,王雨辰,曹铮,徐洋,李倩,胡廷尖..患体表出血症的泥鳅病原菌的鉴定与生物特性分析[J].安徽农业大学学报,2017,44(3):367-372,6.基金项目
浙江省重大科技专项(2013C02009)和国家自然科学基金青年项目(31402328)共同资助. （2013C02009）
