安徽农业大学学报2017，Vol.44Issue(3)：381-387,7.DOI:10.13610/j.cnki.1672-352x.20170524.001
江苏地区渔源嗜水气单胞菌流行病学调查
Epidemiological investigation of piscine source Aermomnas hydrophila in Jiangsu region
摘要
Abstract
The notorious pathogen of Aeromonas hydrophila has high genetic and phenotyping diversity in fisheries aquaculture,which has caused severe mortality of fishes in freshwater fish farming.In this study,we isolated and purified the bacteria from the liver,spleen,kidney,peritoneal effusion and blood of the main reared fishes in Jiangsu region,and tried to unclose the infection prevalence ofA.hydrophila.Ninety-two out of the total isolated bacteria (17.79％) were identified as A.hydrophila using API20E method,while 60 out of the 92 isolates were confirmed as A.hydrophila by PCR method,and the other 32 isolates were detected as Aeronmas spp.The hemolytic test showed that 60 out of the 92 isolates were positive.A.hydrophila was detected in all 12 sampling localities,and the highest positive rate (30.77％) occurred in Dafeng,the lowest in Jintan (10.00％).A.hydrophila was detected between March and October,and it increased since June,reaching the peak between July and September.The assessment ofA.hydrophila infection in different cultured fish species showed an average detection rate of 17.79％.关键词
嗜水气单胞菌/16SrRNA/流行病学Key words
Aermomnas hydrophila/16SrRNA/epidemiology分类
农业科技引用本文复制引用
刘训猛,习丙文,胡翔,王习达,袁锐,吴亚锋,陈辉,方苹..江苏地区渔源嗜水气单胞菌流行病学调查[J].安徽农业大学学报,2017,44(3):381-387,7.基金项目
江苏省水产三新项目(D2015-11 （）
D2013-5)资助. （）
