安徽农业大学学报2017，Vol.44Issue(3)：388-391,4.DOI:10.13610/j.cnki.1672-352x.20170524.003
液相色谱-氢化物发生原子荧光光谱测定鸡肉中4种形态砷化合物
Simultaneous determination of four arsenic species in chicken using liquid chromatography-hydride generation atomic fluorescence spectrometry
摘要
Abstract
An analytical method was developed for the simultaneous determination of four arsenic species including arsenite (As (Ⅲ)),monomethylarsonic acid (MMA),dimethylarsinic acid (DMA),and arsenate (As(V)) in chickens by liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS).The extraction of arsenic species from chicken samples was accomplished in 0.15 mol·L-1 nitric acid with ultrasonic wave.The different arsenic species were separated on a PRP-X 100 anion exchange column and analyzed by HG-AFS.The mobile phase was 5 mmol·L-1 Na2HPO4 and 45 mmol·L-1 KH2PO4 buffer (pH5.92).The separation of four arsenic species were achieved in 8 min.A good linearity was obtained for all four arsenic species,with linear correlation coefficients being greater than 0.999.The detection limit (LODs) of the four species were between 1.0 and 3.0 μg·L-1.The relative standard deviations (RSD) in six replicated measures (100 μg·L-1) varied from 2.0％ to 6.2％.The recovery rate for spiked chicken samples ranged from 82.1％ to 101％.The method is simple and sensitive with a low operation cost and environmental friendly.关键词
砷形态/液相色谱/氢化物发生原子荧光光谱/鸡肉Key words
arsenic species/liquid chromatography/hydride generation atomic fluorescence spectrometry/chicken分类
农业科技引用本文复制引用
陈玎玎,葛高飞..液相色谱-氢化物发生原子荧光光谱测定鸡肉中4种形态砷化合物[J].安徽农业大学学报,2017,44(3):388-391,4.基金项目
2014校学科骨干培育项目(2014XKPY-65)资助. （2014XKPY-65）
