安徽农业大学学报2017，Vol.44Issue(3)：392-397,6.DOI:10.13610/j.cnki.1672-352x.20170524.011
动物源血液中三聚氰胺的液相色谱质谱联用检测方法的建立
The establishment of a detection method for detecting melamine in animal-derived blood
摘要
Abstract
In order to establish a Mass spectrometry method for determination of melamine in animal-derived blood,we investigated the extraction efficiency of various solvents and the purification ability of different SPE columns and evaluated the technical parameters including recovery rate,precision and detection limit.With the 49.5:49.5:1 (methanol:water:acetic acid) solution as an extraction reagent and using MCX column as purifying solid-phase extraction column,the melamine in animal blood samples of recovery ranged from 90.0％ to 110.0％;the precision RSD was in the range of 0.96 ％-4.64％;and the detection limit was 10 μg.kg-1.The result indicated that this optimized method was sensitive and stable,which could be potentially applied to determination of melamine in animal blood samples.关键词
三聚氰胺/质谱联用/动物源血液Key words
melamine/mass spectrometry/animal-derived blood分类
农业科技引用本文复制引用
夏明星,黄均艳,李光满,陈忠祥,孙涛,李应国..动物源血液中三聚氰胺的液相色谱质谱联用检测方法的建立[J].安徽农业大学学报,2017,44(3):392-397,6.基金项目
重庆市应用开发计划项目(cstc2014yykfB80016)和国家认证认可监督管理委员会2014年第三批出入境检验检疫行业标准制修订计划项目(2014B368)共同资助. （cstc2014yykfB80016）
