安徽农业大学学报2017，Vol.44Issue(3)：398-403,6.DOI:10.13610/j.cnki.1672-352x.20170524.010
毛竹PheDof4-1基因克隆及表达分析
Molecular cloning and expression analysis of the PheDof4-1 gene in moso bamboo (Phyllostachys edulis)
摘要
Abstract
Dof (DNA binding with one finger) proteins forming an important transcriptional factor family encoding by multiple genes are involved in gene transcriptional regulation,development and stress-responses in plants.In this study,one of Dofgenes,PheDof4-1 (Gene ID No.PH01000209G1040) was isolated from moso bamboo and the sequence was 1 473 nt in length.The predicted molecular weight of PheDof4-1 protein was 53.2 kDa,and the pI value was 8.05.The qRT-PCR analysis displayed that the PheDof4-1 transcription level in young stem was induced following exposure to cold stress and salt treatment,however,the expression was restrained in the root under drought treatment.PheDof4-1 showed different expression patterns in the leaf under different stress treatments.关键词
毛竹/Dof转录因子/非生物胁迫/表达模式Key words
moso bamboo/Dof transcription factor/abiotic stress/gene expression分类
农业科技引用本文复制引用
刘俊,黄容,程占超,牟少华,李雪平,高健..毛竹PheDof4-1基因克隆及表达分析[J].安徽农业大学学报,2017,44(3):398-403,6.基金项目
国家林业局“948”项目(2014-4-74)和国家自然科学基金(31570673)共同资助. （2014-4-74）
