安徽农业大学学报2017，Vol.44Issue(3)：487-491,5.DOI:10.13610/j.cnki.1672-352x.20170524.016
地涌金莲不同部位外植体多酚氧化酶活性及总酚含量分析
Analysis of dynamic changes of phenols and PPO activity in tissue culture of Musella lasiocarpa
摘要
Abstract
Musella lasiocarpa,which belongs to a monotypic genus,is endemic to southwest of China,including Yunnan and Sichuan province.It is a precious wild flower germplasm resource known for its dignified plant type and beautiful inflorescence.The differences in phenol content and PPO activity between explants of M.lasiocarpa var.Lasiocarpa and its mutant M.lasiocarpa var.Rubribracteata were quantified in order to provide some theoretical basis for selecting appropriate explants in tissue culture.The results showed that there was a significant difference in total phenol content among different parts of the two varieties,of which the total phenol content in the stamen was the highest with 21.42 and 20.64 mg·g-1 in Lasiocarpa and Rubribracteata,respectively.The total phenol content in leaves and veins of M.lasiocarpa var.Rubribracteata was 9.43 and 2.32 mg·g-1,respectively,which was significantly higher than that in M.lasiocarpa var.Lasiocarpa (4.67 and 0.72 mg·g-1,correspondingly).Contrarily,the total phenol content in ovary walls of M.lasiocarpa var.Rubribracteata (4.69 mg·g-1) was significantly lower than that in Lasiocarpa (9.44 mg·g1).There was no significant difference among the other eight parts.For the PPO activity,the leaves and veins of M.lasiocarpa var.Lasiocarpa were 971.5 and 542.8 (0.01△A·g-1·min-1),respectively,which was significantly higher than Rubribracteata (277.2 and 428.5,correspondingly).There was no significant difference among the other parts of the two varieties in the aspect of PPO activity.It is suggested that bracts and male ovary which have relatively low total phenolic content and PPO activity are the ideal explants for tissue culture ofM.lasiocarpa.关键词
地涌金莲/组织培养/褐化/总酚/多酚氧化酶Key words
Musella lasiocarpa/tissue culture/browning/total phenolic content/PPO分类
农业科技引用本文复制引用
杨凯博,舒清态,侯健华,朱丹青..地涌金莲不同部位外植体多酚氧化酶活性及总酚含量分析[J].安徽农业大学学报,2017,44(3):487-491,5.基金项目
国家自然科学基金(31460194)和云南省林学一流学科建设经费共同资助. （31460194）
