安徽农业科学2017，Vol.45Issue(20)：14-17,45,5.
不同诱导子对油樟内生菌次生代谢产物的影响
Effects of Different Inducers on Endophytic Fungi of Cinnamomum longepaniculatum
摘要
Abstract
[Objective]To improve the accumulation of secondary metabolites of endophytic fungi of Cinnamomum longepaniculatum.[Method]Different inducers(oil, water extract and callus from C.longepaniculatum,were added in the culture system of endophytic fungi(YY26,YG42)of C.longepaniculatum to study their effects on the growth of endophytic fungi and their secondary metabolites(1,8-eucalyptol, terpinen-4-ol, α-terpineol).[Result]As for the growth of endophytic fungi, callus from C.longepaniculatum had obvious inhibitory effects on the growth of YY26,but it had promoting effects on the growth of YG42.Oil from C.longepaniculatum had some inhibiting effects on the growth of YY26 and YG42.Water extracts from C.longepaniculatum had no significant effect on the growth of YY26 and YG42.As for the accumulation of secondary metabolites,water extract from C.longepaniculatum had stronger promoting effects on the yield of 1,8-cineole, terpinen-4-ol and α-terpineol of YY26 and YG42, and it had the strongest promoting effects on the yield of 1,8-cineole.When the concentration of water extract was 1.00%,the yield of 1,8-cineole of YY26 was the highest, which was increased than that of control group by 11.80 times.When the concentration of water extract was 2.50%, the yield of 1,8-cineole of YG42 was the highest, which was increased than that of control group by 7.73 times.Callus from C.longepaniculatum had the strongest promoting effects on total volatile of secondary metabolites.When the concentration of callus from C.longepaniculatum was 10.00%,it had the strongest promoting effects on total volatile substances of YY26, which was increased than that of control group by 13.26 times.[Conclusion]The accumulation amount of secondary metabolites of YY26 and YG42 in C.longepaniculatum were improved, which can lay a foundation for its industrialized production in the future.关键词
油樟/内生菌/1,8-桉叶油素/松油烯-4-醇/α-松油醇Key words
Cinnamomum longepaniculatum/Endophytic fungi/1,8-cineole/Terpinen-4-ol/α-terpineol分类
农业科技引用本文复制引用
汪超,谭韵雅,杨胜伟,杨添雁,魏琴,李群..不同诱导子对油樟内生菌次生代谢产物的影响[J].安徽农业科学,2017,45(20):14-17,45,5.基金项目
四川省高校重点实验室项目(2012KFZ001). （2012KFZ001）
