安徽农业科学2017，Vol.45Issue(20)：123-125,3.
不同栽培方式对金线莲中微量元素的影响
Effects of Different Cultivation Methods on Trace Elements in Anoectochilus roxburghii (Wall.) Lindl
摘要
Abstract
[Objective]The research aimed to establish the microwave digestion inductively coupled plasma mass spectrometry (ICP-MS) method for content determination of trace elements in A.roxburghii,which provided the basis for the development of A.roxburghii and the quality standard.[Method]After the treatment of microwave digestion, ICP-MS method was used in the determination.The effects of different cultivars, different cultivation methods and age on the trace elements of A.roxburghii were studied.[Result]The standard liquid working curves of trace elements had good linearity, with the correlation coefficients of more than 0.999.The relative standard deviation(RSD) of precision was less than 4.4% and RSD of repeatability was less than 5.8%.The average recovery was 89.4%-114.2%,with RSD of less than 4.8%.Different varieties of A.roxburghii presented great differences in contents of inorganic elements, with high content of Ca, Mg, Fe, Mn, Zn.[Conclusion]The content of trace elements increase along with the prolonged cultivation cycle.The method is fast and accurate, sensitive and stable, good reproducibility.It can be used for the simultaneous determination of trace elements in A.roxburghii.关键词
金线莲/微量元素/ICP-MS/微波消解/栽培方式Key words
Anoectochilus roxburghii (Wall.) Lindl/Trace elements/Inductively coupled plasma mass spectrometry(ICP-MS)/Microwave digestion/Cultivation methods分类
农业科技引用本文复制引用
程伟青,吴水华,邓笑治..不同栽培方式对金线莲中微量元素的影响[J].安徽农业科学,2017,45(20):123-125,3.基金项目
福建省生物医药产业中试与检测技术公共服务平台(2014Y2008) （2014Y2008）
福建省教育厅项目(JB14165). （JB14165）
