重庆医学2017，Vol.46Issue(20)：2747-2750,4.DOI:10.3969/j.issn.1671-8348.2017.20.004
自体富血小板血浆对兔膝关节骨性关节炎的治疗效果观察
Observation on effect of autologous platelet-rich plasma in treating rabbit knee osteoarthosis
摘要
Abstract
Objective To investigate the treatment mechanism and effect of platelet-rich plasma(PRP) in treating rabbit knee osteoarthosis(KOA).Methods Thirty-two New Zealand adult clean class rabbits were randomly divided into the blank control group(A),model control group(B),PRP treatment group(C) and sodium hyaluronate treatment group(D).The group A conducted the simulated model construction,while the group B,C and D established the KOA rabbit model by using the Hulth method.After establishing the animal model,the group C was given PRP 0.5mLl by knee articular cavity injection,once every 3 weeks,twice in total;the group D was given sodium hyaluronate by knee articular cavity injection,once per week for 5 continuous weeks.The group A and B were injected with equal amount of normal saline at the same time point in the group C.The histological structure of articular cartilage,cell number,integrity of tidal line,and toluidine blue staining were observed by common optical microscope.The differences of Mankin′s scores were compared among various groups.The proper amount of knee joint fluid was collected for measuring the arachidonic acid expression in each group.Results The structure and morphology of articular cartilage in the group A were normal without obvious damage,while which in the group B,C and D had different degrees of damage,especially the cartilage structure in the group B had greater changes.Although the group B and C had the morphological and structure change of articular cartilage,but which was close to the cartilage structure in the group A.The Mankin′s score in the group A was lowest,while which in the group B was highest,which in the group C and D was significantly decreased after intervention treatment;the difference between the group B with the group C and D had statistical significance(P<0.05).The difference between the group C and D had statistical significance(P<0.05).The arachidonic acid level in the group A was lowest and which in the group B was highest,which in the group C and group D ranged between the group A and B,moreover the group C was lower than the group D (P<0.05).Conclusion PRP has obvious therapeutic and alleviated effect in treating KOA.关键词
膝关节/骨性关节炎/运动医学/富血小板血浆/花生四烯酸Key words
knee joint/osteoarthrosis/sports medicine/platelet-rich plasma/arachidonic acid分类
医药卫生引用本文复制引用
咸杰,何本祥,吴骁,檀亚军..自体富血小板血浆对兔膝关节骨性关节炎的治疗效果观察[J].重庆医学,2017,46(20):2747-2750,4.基金项目
四川省科技厅重点项目(2016JY0052) （2016JY0052）
四川省科技厅支撑项目(2014SZ0003) （2014SZ0003）
成都体育学院硕士点建设基金项目(16SSZX01). （16SSZX01）
