重庆医学2017，Vol.46Issue(21)：2892-2894,3.DOI:10.3969/j.issn.1671-8348.2017.21.004
下调PI3K/Akt信号通路对抑制喉癌细胞生长的影响
Effects of down-regulation of PI3K/Akt signaling pathway on growth of laryngeal cancer cells
摘要
Abstract
Objective To investigate the effect of potassium channel blocker tetraethtylamine (TEA) on the proliferation and apoptosis of human laryngeal carcinoma cells (Hep-2) through the PI3K/Akt signaling pathway.Methods TEA acted on HEp-2.The methyl thiazolyl blue (MTT) method was used to determine the Hep-2 cell activity and the proliferation inhibition rate of TEA on Hep-2 was calculated;the Hoechest33258 staining was used to detect cell apoptosis,and the Hep-2 apoptosis rate was detected by flow cytometry (FCM) assay.Results After inoculation by 5,10,20 mmol/L TEA,the Hep-2 cell proliferation inhibition rate reached 12.573%,31.385% and 56.132%,respectively.After 96 h Hep-2 cell action,the cellular apoptosis rates were (41.64±2.67)%,(58.76±4.32)% and (72.65±6.54)% respectively,the inhibition rate and apoptosis rate in the TEA treating group were significantly higher than those in the non-TEA treating group,the differences were statistically significant (P<0.05).Conclusion TEA can inhibit the proliferation and in vivo growth of human laryngeal carcinoma cells and promotes the apoptosis of laryngeal cancer cells.关键词
钾通道阻滞剂/喉肿瘤/上皮细胞/PI3K-Akt/细胞凋亡Key words
potassium channel blockers/laryngeal neoplasms/epithelial cells/PI3K/Akt/apoptosis分类
医药卫生引用本文复制引用
王慧敏,余文发,周航,卢振民..下调PI3K/Akt信号通路对抑制喉癌细胞生长的影响[J].重庆医学,2017,46(21):2892-2894,3.基金项目
河南省重点科技攻关项目(152102310355). （152102310355）
