重庆医学2017，Vol.46Issue(21)：2898-2901,4.DOI:10.3969/j.issn.1671-8348.2017.21.006
阿奇霉素通过TRAF6/NF-κB/VEGF信号通路抑制哮喘大鼠气道平滑肌细胞增殖
Azithromycin inhibits proliferation of rat airway smooth muscle cells though TRAF6/NF-κB/VEGF signaling pathway
李小莉 1王威 1郭纪元 2郭省香1
作者信息
- 1. 西安市第三医院儿科 710018
- 2. 西安市儿童医院儿科 710002
- 折叠
摘要
Abstract
Objective To explore the mechanism of azithromycin (AZM) for inhibiting the proliferation of rat airway smooth muscle cells (ASMCs).Methods Thirty Sprague-Dawley (SD) rats were divided into the control group,asthma model group and AZM group.The rat model of asthma was established by ovalbumin (OVA) sensitization and stimulation in vitro.The airway related parameters of rat lung tissue were determined by using the medical image analysis system.Primary passage ASMCs were isolated and cultured using the tissue-sticking method,and the vascular endothelial growth factor (VEGF) overexpression vector or tumor necrosis factor receptor-associated factor 6 (TRAF6) overexpression vector was transfected into ASMCs in the AZM group.The protein levels of VEGF,NF-κB p65 and TRAF6 were detected by Western blotting,and the proliferation of ASMCs was evaluated by CCK-8 kit.Results AZM significantly inhibited the increase of thickness of total airway wall,thickness of inner airway wall and thickness of airway smooth muscle layer in asthma rats (P<0.05),also significantly inhibited the proliferation of ASMCs in the asthma model group (P<0.05).AZM significantly inhibited the protein expression of VEGF and NF-κB p65 induced by asthma (P<0.05),and the overexpression of VEGF significantly reduced the inhibiting effects of AZM on proliferation of ASMCs (P<0.05).AZM significantly inhibited the high expression of TRAF6 induced by asthma (P<0.05),and the overexpression of TRAF6 significantly reduced the inhibiting effects of AZM on expression of VEGF and NF-κB p65 as well as proliferation of ASMCs (P<0.05).Conclusion AZM can suppress the proliferation of ASMCs,its partial mechanism may be realized through inhibiting TRAF6/NF-κB/VEGF signaling pathway.关键词
阿奇霉素/哮喘/气道平滑肌细胞/TRAF6/NF-κB/血管内皮生长因子Key words
azithromycin/asthma/airway smooth muscle cell/TRAF6/NF-κB/vascular endothelial growth factor分类
医药卫生引用本文复制引用
李小莉,王威,郭纪元,郭省香..阿奇霉素通过TRAF6/NF-κB/VEGF信号通路抑制哮喘大鼠气道平滑肌细胞增殖[J].重庆医学,2017,46(21):2898-2901,4.
