大连工业大学学报2017，Vol.36Issue(4)：235-239,5.
以菊粉为底物全细胞催化生产甘露醇
Q819AThe whole cell catalytic production of mannitol using inulin as substrate
摘要
Abstract
A whole-cell biotransformation system E.coli BL21(DE3)/pRSFDuet-mdh-fdh pZY507glf for mannitol production were established through the mannitol dehydrogenase (mdh) derived from Leuconostoc mesenteroides and the formate dehydrogenase gene (fdh) derived from Mycobacterium vaccae pRSFDuet-mdh-fdh with the glucose facilitator protein (glf) pZY507glf co-expressed in E.coli BL21(DE3).The yield of mannitol could reach to 0.91 g/g under the optimum conditions of pH 6.5 at 30 ℃.The yield of mannitol could reach to 0.93 g/g by whole catalyst with high activity inulinase.关键词
甘露醇/生物转化法/菊粉/全细胞催化Key words
mannitol/biotransformation/inulin/whole cell catalysis分类
轻工纺织引用本文复制引用
罗希,曹海龙,张卉妍,李悝悝,张春枝..以菊粉为底物全细胞催化生产甘露醇[J].大连工业大学学报,2017,36(4):235-239,5.基金项目
国家高技术研究发展计划(863计划)项目(2014AA093511). （863计划）
