大连工业大学学报2017，Vol.36Issue(4)：250-254,5.
人参皂苷酶Ⅲ型转化人参皂苷Rc制备稀有皂苷C-Mc
Preparation of minor ginsenoside C-Mc from ginsenoside Rc using special ginsenosidase type-Ⅲ
摘要
Abstract
To study the enzymatic conversion from ginsenoside Rc to the rare ginsenoside C-Mc with high active, the ginsenosidase Ⅲ was expressed from recombinant Escherichia coli C41 and its enzymatic transformation processes were explored.The 3-O-glucopyranoside end of ginsenoside Rc was hydrolyzed to C-Mc1 by ginsenosidase Ⅲ, then C-Mc1 was transformed to C-Mc.4.8 g ginsenoside Rc was hydrolyzed to 2.8 g products by ginsenosidase Ⅲ, of which purity could reach to 90% determined by HPLC.The result of NMR showed that its structure was 20-O-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyl]-20(S)-protopanaxadiol, that was C-Mc.关键词
稀有人参皂苷/人参皂苷酶Ⅲ型/高效液相色谱/核磁共振Key words
rare ginsenoside/ginsenosidase Ⅲ/HPLC/NMR分类
轻工纺织引用本文复制引用
李冠亨,刘春莹,徐龙权,林完泽,鱼红闪..人参皂苷酶Ⅲ型转化人参皂苷Rc制备稀有皂苷C-Mc[J].大连工业大学学报,2017,36(4):250-254,5.基金项目
"重大新药创制"科技重大专项(2012ZX09503001-003) （2012ZX09503001-003）
国家高端外国专家项目(GDT20152100019). （GDT20152100019）
