分析测试学报2017，Vol.36Issue(1)：31-36,6.DOI:10.3969/j.issn.1004-4957.2017.01.005
自组装管尖固相萃取/超高效液相色谱-高分辨质谱法测定水果中的15种酸性农药
Determination of 15 Acidic Pesticides in Fruit by Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry with a Self-assembly Pipette Tip Solid-phase Extraction
摘要
Abstract
A self-assembly pipette tip solid-phase extraction (PTSPE)/high-resolution benchtop Q Exactive mass spectrometric(HRMS) method for the analysis of 15 acidic pesticides in fruit was established.The samples were first extracted with 1％ formic acid in acetonitrile,and then the extracts were cleaned up using PTSPE procedure with the adsorbent of PEP and HILIC.The chromatographic analysis was performed on a T3 column.And the qualitative and quantitative analyses were performed under tSIM/ddMS2 mode by high resolution mass spectrometry.The internal standard calibration was used for quantification,and the method showed a good linearity (r2 ＞ 0.99) in a certain concentration range for the 15 acidic pesticides.The limits of quantitation(LOQ) were between 0.2 μg/kg and 10 μg/kg.The recoveries of the analytes in apples,cherry tomatoes and strawberries samples at spiked levels of LOQ,10 μg/kg and 100 μg/kg ranged from 73.5％ to 115.9％,with relative standard deviations(RSDs) of 1.0％-8.3％.The results showed that the method was sensitive and accurate,and was suitable for the analysis of acidic pesticides in fruit.关键词
管尖固相萃取/超高效液相色谱-高分辨质谱法/水果/酸性农药Key words
pipette tip solid-phase extraction(PTSPE)/ultra high performance liquid chromatography-high resolution mass spectrometry(UPLC-HRMS)/fruit/acidic pesticides分类
化学化工引用本文复制引用
邱楠楠,董桂贤,陈达炜,周爽,赵云峰..自组装管尖固相萃取/超高效液相色谱-高分辨质谱法测定水果中的15种酸性农药[J].分析测试学报,2017,36(1):31-36,6.基金项目
国家食品安全风险评估中心高层次人才队伍建设523项目 （）
