分析测试学报2017，Vol.36Issue(1)：47-53,7.DOI:10.3969/j.issn.1004-4957.2017.01.008
基于分散固相萃取液相色谱-串联质谱法测定大米中8种真菌毒素
Determination of Eight Mycotoxins in Rice by Dispersive Solid Phase Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A rapid method was developed for the determination of HT-2 toxin,T-2 toxin,fumonisin B1,fumonisin B2,zearalenone,ochratoxin A,deoxynivalenol and aflatoxins B1 in rice.Three dispersive solid phase extraction sample preparation methods,including QuEChERS method,EMR-lipid method and DisQuE method,were compared for the determination of 8 mycotoxins in rice.The recoveries of the three extraction methods for the toxins were studied.The method of afterextraction addition was used to evaluate the matrix effects for mycotoxins in rice by liquid chromatography-tandem mass spectrometry(LC-MS/MS).As a result,the QuEChERS method was not suitable for the analysis of fumonisin B1,fumonisin B2 and ochratoxin A.The EMR-lipid methods could not overcome the matrix effects of zearalenone and ochratoxin A in rice.In this study,mycotoxins were extracted by DisQuE method,and analysed by LC-MS/MS under the optimized condition with monitoring 16 pairs of MS/MS transitions of precursor ions(two for each mycotoxin) in one single run.Recoveries at three fortified levels in rice ranged from 70.0％ to 124.1％ with relative standard deviations of 0.9％-16.9％.The limits of detection(S/N≥3) were in the range of 1.2-60.0 μg/kg.The method was proved to be highly efficient,robust and sensitive,and was suitable for the simultaneous analysis of mycotoxins in rice.关键词
QuEChERS方法/EMR-lipid方法/DisQuE方法/LC-MS/MS/真菌毒素/大米Key words
QuEChERS method/EMR-lipid method/DisQuE method/liquid chromatography-tandem mass spectrometry(LC-MS/MS)/mycotoxins/rice分类
化学化工引用本文复制引用
孙伟华,杨欢,曹赵云,马有宁,秦美玲,柴爽爽,陈铭学..基于分散固相萃取液相色谱-串联质谱法测定大米中8种真菌毒素[J].分析测试学报,2017,36(1):47-53,7.基金项目
国家农产品质量安全风险评估项目(GJFP2016001001) （GJFP2016001001）
中央级公益性科研院所基本科研业务费专项资金项目(2014RG006) （2014RG006）
