分析测试学报2017，Vol.36Issue(1)：101-105,111,6.DOI:10.3969/j.issn.1004-4957.2017.01.017
酪氨酸四肽与ct-DNA的相互作用
Studies on Interaction of Tyr-Tyr-Tyr-Tyr with ct-DNA
摘要
Abstract
A model peptide,Tyr-Tyr-Tyr-Tyr(YYYY),was synthesized by Fmoc solid-phase methods,purified and separated by reverse phase high performance liquid chromatography (RP-HPLC),and characterized by ESI-MS analysis.The interaction of peptide with calf thymus DNA (ct-DNA) was investigated by UV-Vis absorption spectra,circular dichroism (CD) and fluorescence spectra.The results showed that,with the increase of peptide concentration,there were a hyperchromic effect on UV spectra,a remarkable fluorescence quenching and a red shift of CD.It is possible that Tyr-Tyr-Tyr-Tyr and Tyr might interact with ct-DNA via the groove binding mode.The interaction between Tyr-Tyr-Tyr-Tyr and ct-DNA,compared with that between Tyr and ct-DNA,is more stronger.关键词
酪氨酸四肽/ct-DNA/相互作用/Fmoc固相合成法Key words
Tyr-Tyr-Tyr-Tyr/ct-DNA/interaction/Fmoc solid-phase methods分类
化学化工引用本文复制引用
孙志杰,吕名秀,卢奎,段冰潮,刘广斌..酪氨酸四肽与ct-DNA的相互作用[J].分析测试学报,2017,36(1):101-105,111,6.基金项目
国家自然科学基金项目(21572046) （21572046）
河南省教育厅自然科学基金项目(14A150017) （14A150017）
