分析测试学报2017，Vol.36Issue(1)：117-121,5.DOI:10.3969/j.issn.1004-4957.2017.01.020
猪肉中β-受体激动剂AlphaLISA检测方法的建立
Quantitative Determination of β-Agonists in Pork Using the Amplified Luminescent Proximity Homogeneous Assay
摘要
Abstract
An amplified luminescent proximity homogeneous assay (AlphaLISA) was developed for the determination of four kinds of β-agonists (clenbuterol,salbutamol,brombuterol,terbutaline) in pork.The analytes were stepwise extracted with ammonium acetate and β-glucuronidase/sulfatase,the extract was columned and concentrated to dryness,redisolved in buffer.Samples were reacted with botinylated antigen,antibody,donor beads and acceptor beads,and detected by AlphaLISA with matrix standard curve.As a result,there are good linear relationships in the concentration range of 0.1-500 ng/mL,with correlation coefficients larger than 0.98.The cross-reactivities for clenbuterol,brombuterol,terbutaline were 143.3％,179.2％ and 95.6％,respectively,and the limits of detection(LOD) were 0.03-0.08 ng/mL.The average recoveries of four compounds at spiked levels of 0.5,10,20 μg/kg were in the range of 82.5％-115.9％ with relative standard deviations (RSD) not less than 12.0％.The method is simple,rapid and accurate,and is suitable for the detection and screening of3-agonists in pork.关键词
AlphaLISA/β-受体激动剂/猪肉Key words
AlphaLISA/β-agonists/pork分类
化学化工引用本文复制引用
龚倩,郗存显,聂福平,曹淑瑞,唐柏彬,王国民,陈冬东,母昭德..猪肉中β-受体激动剂AlphaLISA检测方法的建立[J].分析测试学报,2017,36(1):117-121,5.基金项目
国家质量监督检验检疫总局科技计划项目(2015IK333) （2015IK333）
质检公益性行业科研专项项目(201210086) （201210086）
