分析测试学报2017，Vol.36Issue(1)：145-150,6.DOI:10.3969/j.issn.1004-4957.2017.01.025
亲水作用色谱在糖类化合物分析中的应用
Application of Hydrophilic Interaction Chromatography in Analysis of Carbohydrates
摘要
Abstract
The analysis of carbohydrate structure and composition plays a vital role in the study of structure-activity relationship of carbohydrates.Hydrophilic interaction chromatography (HILIC) could be used to separate polar compounds like carbohydrates with satisfactory resolution.The stationary phase and retention mechanism of HILIC column commonly used in carbohydrates analysis were introduced.The effects of strong elution solvent ratio,mobile phase pH value,buffer salt concentration and column temperature on separation were also discussed.Application examples in analysis of monosaccharide composition,disaccharide composition of glycosaminoglycans,polymerization degree of oligosaccharides,glycosides,glycolipids,sugar alcohols and N-/O-glycans by HILIC were illustrated in this paper.关键词
亲水作用色谱/固定相/保留机制/糖类分析/综述Key words
hydrophilic interaction chromatography/stationary phase/retention mechanism/carbohydrate analysis/review分类
化学化工引用本文复制引用
蒲江华,赵峡,韩文伟,白明月..亲水作用色谱在糖类化合物分析中的应用[J].分析测试学报,2017,36(1):145-150,6.基金项目
国家自然科学基金-山东省政府联合资助项目(U1406402) （U1406402）
国家“十二五”科技支撑计划(2013BAB01B02) （2013BAB01B02）
